外源猪生长激素基因在金鱼体内的整合与表达研究

自1982年Palmiter^1]等人给小鼠受精卵雄原核注射大鼠生长激素基因培养成功“超级”鼠以来，由于人们认识到转基因技术导致动植物品种定向改良以及利用转基因动物作为生物反应器，大量合成和分泌贵重而安全的基因工程产品，因此转基因动物技术得到了迅速发展，相继开展了兔、鱼、猪、鸡、牛、羊等动物的转基因研究，并取得了一定的结果^2，3，4]。但是在上述具有经济价值的高等脊椎动物中从事转基因研究，成本高、操作困难，而金鱼属于低等脊椎动物．具有产卵多、易获得同步卵、可控制体外受精和体外发育等特点，因而成为转基因动物研究的方便材料。生长激素是动物脑下垂体前叶分泌的单链多肽⑸，生理功能是促进碳水化合物代谢及核酸、蛋白质合成，整体效应表现为动物生长。本文以金鱼为实验材料，探讨猪生长激素基因导入其受精卵后整合、表达以及生物效应等问题，为进一步研究外源基因在高等动物内整合和表达调控机理提供参考。

The Study on Integration and Expression of Porcine Growth Hormone Genein Transgenic Goldfish

A linear recombinant plasmid, consisting of sheep metallothionein gene promoter and porcine growth hormone gene, was microinjected into goldfish fertilized eggs. The experimental goldfishes were detected and screened by using dot blotting and Southern blotting to obtain foreig gene integration positive transgenic goldfish, which were further used for mating each other. F1 and F2 generations were detected by using PCR, respectively. Our data, including PGH expression level in transgenic goldfish serum detected radioimmunoassy, showed that foreign genes have been integrated and expressed in some transgenic goldfish, and passed into next generation through sex reproduction.