| 猪细小病毒VP2蛋白在干酪乳杆菌表面的表达 |
| |
| 引用本文： | 徐义刚,崔丽春,马广鹏,唐丽杰,葛俊伟,夏春丽,乔薪媛,赵丽丽,李一经.猪细小病毒VP2蛋白在干酪乳杆菌表面的表达[J].生物工程学报,2007,23(2):315-318. |
| |
| 作者姓名： | 徐义刚 崔丽春 马广鹏 唐丽杰 葛俊伟 夏春丽 乔薪媛 赵丽丽 李一经 |
| |
| 作者单位： | 1. 东北农业大学动物医学院,哈尔滨,150030
2. 东北林业大学,哈尔滨,150040 |
| |
| 摘 要： | 将编码猪细小病毒主要免疫保护性抗原VP2基因插入干酪乳杆菌细胞表面表达载体pPG中,构建了重组表达载体pPG-VP2,将其电转化干酪乳杆菌Lactobacillus casei 393,获得了表达猪细小病毒VP2蛋白的重组干酪乳杆菌系统,经2%乳糖在MRS培养基中的诱导表达,SDS-PAGE检测表明,有约74kD蛋白得到了表达,表达蛋白的大小与理论值相符。Western-blot结果分析表明,表达的蛋白可被鼠源PPV抗血清所识别,间接免疫荧光实验结果表明,所表达的蛋白能够在干酪乳杆菌菌体表面检测到。
|
| 关 键 词： | 猪细小病毒 VP2蛋白 干酪乳杆菌 表面表达 |
| 文章编号： | 1000-3061(2007)02-0315-04 |
| 修稿时间： | 09 27 2006 12:00AM |
The Surface Display of Porcine Parvovirus VP2 Protein in Lactobacillus casei |
| |
| XU Yi-Gang,CUI Li-Chun,MA Guang-Peng,TANG Li-Jie,GE Jun-Wei,XIA Chun-Li,QIAO Xin-Yuan,ZHAO Li-Li and LI Yi-Jing.The Surface Display of Porcine Parvovirus VP2 Protein in Lactobacillus casei[J].Chinese Journal of Biotechnology,2007,23(2):315-318. |
| |
| Authors: | XU Yi-Gang CUI Li-Chun MA Guang-Peng TANG Li-Jie GE Jun-Wei XIA Chun-Li QIAO Xin-Yuan ZHAO Li-Li and LI Yi-Jing |
| |
| Institution: | Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;Northeast Forestry University, Harbin 150040, China;Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China |
| |
| Abstract: | Lactobacillus casei 393 was selected as a bacterial carrier for the ex pression of Porcine Parvovirus(PPV)protective antigen VP2 protein.The gen e en coding PPV VP2 protein was cloned into the Lactobacillus casei surface expre ssio n vector pPG,and then the constructed recombinant vector pPG-VP2 was electrotr a nsformed into Lactobacillus casei 393 generating the recombinant system pPG-VP2/L.casei393 expressing PPV VP2 pr otein.The recombinant stra i n was induced by 2% Lactose in MRS and about 74kD protein was detected with SDS-PAGE.The result of Western-blot indicated that the expressed protein possessed the antigenic specificity which could be recognized by mouse anti-PPV serum.Th e indirect immunofluorescent test showed that the expressed protein was secreted on the cell surface Lactobacillus casei. |
| |
| Keywords: | PPV VP2 protein Lactobacillus casei 393 surface display |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《生物工程学报》浏览原始摘要信息 |
| 点击此处可从《生物工程学报》下载免费的PDF全文 |
|
