| 表达鸡传染性法氏囊病毒VP2蛋白的干酪乳杆菌免疫保护效力 |
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| 引用本文： | 林红丽,侯申达,王嵩,王宇鹏,栾云艳,侯喜林.表达鸡传染性法氏囊病毒VP2蛋白的干酪乳杆菌免疫保护效力[J].生物工程学报,2014,30(11):1679-1690. |
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| 作者姓名： | 林红丽 侯申达 王嵩 王宇鹏 栾云艳 侯喜林 |
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| 作者单位： | 1 黑龙江八一农垦大学动物科技学院，黑龙江 大庆 163319;2 中山大学医学院，广东 广州 510000;1 黑龙江八一农垦大学动物科技学院，黑龙江 大庆 163319;1 黑龙江八一农垦大学动物科技学院，黑龙江 大庆 163319;1 黑龙江八一农垦大学动物科技学院，黑龙江 大庆 163319;1 黑龙江八一农垦大学动物科技学院，黑龙江 大庆 163319 |
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| 基金项目： | 黑龙江省研究生创新科研项目(No. YJSCX2013-11BYND)资助。 |
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| 摘 要： | 利用干酪乳杆菌作为传染性法氏囊病毒(IBDV)VP2抗原传递系统,探讨口服雏鸡的免疫次数、免疫剂量、免疫途径和攻毒保护效果。用pLA-VP2重组干酪乳杆菌对5日龄雏鸡进行二次和三次免疫,并设108、109、1010 CFU/mL的重组干酪乳杆菌组,间接ELISA检测血清IgG和小肠洗液sIgA,末免后7 d攻毒,计算保护效果。根据确定的2次免疫和109 CFU/mL免疫剂量免疫5日龄雏鸡,分别口服、滴鼻/点眼pLA-VP2/L.casei,口服、肌注商品活苗及口服pLA/L.casei和PBS为对照,监测IgG和sIgA抗体水平;末免后7 d检测脾淋巴细胞增殖情况并攻毒,7 d后剖检,观察法氏囊损伤程度并记录病变得分和保护率。结果表明各组的特异性sIgA、IgG抗体水平显著高于对照组(P0.01);口服pLA-VP2/L.casei组的淋巴细胞刺激指数显著高于其他组(P0.01),保护率高达83.3%,免疫保护效果优于滴鼻/点眼组。因此,构建的重组干酪乳杆菌的安全性优于商品活苗,可以作为IBDV候选疫苗。
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| 关 键 词： | 传染性法氏囊病毒(IBDV) VP蛋白 重组干酪乳杆菌 黏膜免疫 免疫次数 免疫剂量 免疫途径 保护率 |
| 收稿时间： | 2014/1/10 0:00:00 |
Immunogenicity of recombinant Lactobacillus casei expressing VP2 protein of infectious bursal disease virus in chickens |
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| Hongli Lin,Shenda Hou,Song Wang,Yupeng Wang,Yunyan Luan and Xilin Hou.Immunogenicity of recombinant Lactobacillus casei expressing VP2 protein of infectious bursal disease virus in chickens[J].Chinese Journal of Biotechnology,2014,30(11):1679-1690. |
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| Authors: | Hongli Lin Shenda Hou Song Wang Yupeng Wang Yunyan Luan and Xilin Hou |
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| Institution: | 1 College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China;2 Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510000, Guangdong, China;1 College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China;1 College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China;1 College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China;1 College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China |
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| Abstract: | In order to determine immunogenicity and protective effect in chickens, we used the IBDV (Infectious bursal disease virus)-Vp2/Lactobacillus casei as antigen transfer system. First, the immunized and control chickens were challenged by IBDV/DQ at lethal dose to determine the protective ratio. Second, chickens were orallyand intranasally vaccinated twice with 109 CFU/mL pLA-VP2/L. casei, pLA/L. casei and PBS as negativecontrol and commercial vaccine as positive control. The bursa injury and the lesion score wererecorded post challenge. The level of specific IgG and sIgA in pLA-VP2/L. casei and positive control groups was significantly higher than that in negativecontrol groups. The protection efficacy in pLA-VP2/L. casei oral group was higher than that inintranasal group. The SI. of pLA-VP2/L. casei oral group was significant higher than other groups. The lesion score indicated the pLA-VP2/L. casei was safer than commercial vaccine for bursa. Collectively, the pLA-VP2/L. casei could be a vaccine candidate for IBDV. |
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| Keywords: | infectious bursal disease virus VP2 protein recombinant Lactobacillus casei mucosalimmunity immunization times immunization dosage immunization wags protective ratio |
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