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| 一株假单胞菌(Pseudomonas sp.)石油脱有机氮分析 | |
| 引用本文： | 李力,许平,史权.一株假单胞菌(Pseudomonas sp.)石油脱有机氮分析[J].生物工程学报,2008,24(6):1080-1084. |
| 作者姓名： | 李力 许平 史权 |
| 作者单位： | 1. 山东大学环境科学与工程学院,济南,250100 2. 山东大学微生物技术国家重点实验室,济南,250100 3. 中国石油大学(北京)重质油国家重点实验室,北京,102200 |
| 基金项目： | 国家自然科学基金(No. 20377026, 20590368), 国家高技术研究发展计划 (863) (No. 2004AA649160)资助。 |
| 摘 要： | 为了探讨咔唑降解菌在石油中的脱氮性能, 从研究咔唑降解菌Pseudomonas sp. XLDN4-9在双液相系统中降解咔唑的性能出发, 分别考察了XLDN4-9休止细胞体系对原油、润滑油及柴油的脱氮效果, 并借助于GC-MS分析了柴油中咔唑及其衍生物的降解状况。结果表明, 正十四烷-水系统有利于咔唑的降解; 以低氮柴油代替正十四烷, 2 g/L咔唑可在15 h内降解95.2%; XLDN4-9休止细胞体系对原油、润滑油、柴油均有显著脱氮效果。在柴油脱氮过程中, 发现3 天后, 99%的咔唑被降解, 四种单甲基咔唑的降解率为63.4%~87.6%, 二甲基咔唑共降解了15%。 |
| 关 键 词： | 咔唑 生物降解 石油 生物脱氮 |
| 收稿时间： | 2008/3/14 0:00:00 |
Denitrogenation of Petroleum by a Pseudomonas sp. |
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| Li Li,Ping Xu and Quan Shi.Denitrogenation of Petroleum by a Pseudomonas sp.[J].Chinese Journal of Biotechnology,2008,24(6):1080-1084. | |
| Authors: | Li Li Ping Xu and Quan Shi |
| Institution: | School of Environmental Science and Engineering, Shandong University, Jinan 250100, China;State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China;State Key Laboratory of Heavy Oil Processing, University of Petroleum, Beijing 102200, China |
| Abstract: | Biodenitrogenation of petroleum oil was investigated by a previously isolated carbazole-degrader Pseudomonas sp. XLDN4-9. In a tetradecane-aqueous phase system, biodegradation of carbazole was enhanced by the presence of n-tetradecane. And strain XLDN4-9 was capable of absorbing 95.2% of 2 g/L carbazole dissolved in diesel within 15 hours. Significant denitrogentation of crude oil, diesel and lubricanting oil was detected by strain XLDN4-9. Removal of carbazole, methylcarbazole, and dimethylcarbazole in diesel was confirmed by using GC-MS. After 3 days, 99% of carbazole and 15% of dimethyl carbazole was degraded. And the removal rate of 1-, 2-, 3-, and 4-methyl carbazole was determined to be 63.4%, 87.6%, 78.4%, and 66.5% respectively. |
| Keywords: | carbazole biodegradation petroleum biodenitrogenation |
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