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| 禽IL-2与传染性法氏囊VP2融合蛋白免疫学特性 | |
| 引用本文： | 王臣,赵战勤,张春杰,刘一尘,丁轲,李银聚,程相朝,陈溥言.禽IL-2与传染性法氏囊VP2融合蛋白免疫学特性[J].生物工程学报,2010,26(4):476-482. |
| 作者姓名： | 王臣 赵战勤 张春杰 刘一尘 丁轲 李银聚 程相朝 陈溥言 |
| 作者单位： | 1. 河南科技大学,动物传染病与微生物实验室,洛阳,471003;南京农业大学,农业部动物疫病与诊断重点实验室,南京,210095 2. 河南科技大学,动物传染病与微生物实验室,洛阳,471003 3. 南京农业大学,农业部动物疫病与诊断重点实验室,南京,210095 |
| 基金项目： | 河南省科技攻关项目 (No. 0424050010)，河南科技大学博士基金项目 (No. 09001397) 资助。 |
| 摘 要： | 为研究禽细胞因子IL-2与IBDV主要保护性抗原VP2基因融合蛋白的免疫学特性,将重组的rVP2-IL-2融合蛋白免疫鸡,通过IBDV-VP2 ELISA抗体效价、抗体亚型(IgG1和IgG2a)、淋巴细胞增殖、INF-γ和IL-4细胞因子的分泌水平、中和抗体以及动物攻毒试验检测评价其对鸡体免疫水平的影响。抗体滴度测定和淋巴细胞增殖试验结果显示,rVP2-IL-2融合蛋白免疫鸡体的体液和细胞免疫应答水平均明显高于单独的VP2蛋白免疫组。抗体亚型测定结果显示,rVP2-IL-2融合蛋白免疫组鸡体能产生一个平衡的IgG1和IgG2a抗体反应。细胞因子ELISA试验结果表明rVP2-IL-2融合蛋白能有效平衡Th1(γ-IFN)和Th2(IL-4)类型的细胞免疫反应。动物攻毒试验rVp2-IL-2融合蛋白免疫组鸡体获得了85%的保护率,表明构建的rVP2-IL-2融合蛋白对IBDV的攻击具有较好的免疫保护作用。本研究为进一步研制IBD高效的基因工程疫苗奠定了基础。 |
| 关 键 词： | 传染性法氏囊，VP2基因，IL-2，融合蛋白 |
| 收稿时间： | 2009/10/10 0:00:00 |
Influence of fusion protein of IBDV VP2 and chicken Interleukin-2 on immune response in chicken |
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| Chen Wang,Zhanqin Zhao,Chunjie Zhang,Yichen Liu,Ke Ding,Yinju Li,Xiangchao Cheng and Puyan Chen.Influence of fusion protein of IBDV VP2 and chicken Interleukin-2 on immune response in chicken[J].Chinese Journal of Biotechnology,2010,26(4):476-482. | |
| Authors: | Chen Wang Zhanqin Zhao Chunjie Zhang Yichen Liu Ke Ding Yinju Li Xiangchao Cheng and Puyan Chen |
| Institution: | College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China; Key Laboratory of Animal Disease Diagnosis and Immunology, Nanjing Agricultural University, Nanjing 210095, China;College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China;College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China;College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China;College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China;College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China;College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China;Key Laboratory of Animal Disease Diagnosis and Immunology, Nanjing Agricultural University, Nanjing 210095, China |
| Abstract: | In order to research immunogenicity of the recombinant rVP2-IL-2 fusion protein,we obtained the rVP2-IL-2 fusion protein using Pichia pastoris expression system,and then evaluated its potential to induce immune responses in chicken.The effect was determined in the form of protective anti-IBDV VP2 titers,antibodies(IgG1 and IgG2a),lymphocyte proliferation,the levels of interferon-7 and interleukin-4 cytokines,and challenge experiment.Antibody titers and proliferation lymphocyte level suggested that the fusion protein could elicit specific humoral immune and cellular immune responses.antibody sub-type results indicated that the rVP2-IL-2 fusion protein induced secretion both of IgGl and IgG2a.The seem result elicited from cytokines ELISA test,secretion of both ofThl(γ-IFN)and Th2(IL-4)were induced by the rVP2-IL-2 fusion protein.Challenge experiment result shown that chicken immunized the rVP2-IL-2 fusion protein obtained 85%protection.These results confirm that the fusion protein enhances the protection against IBDV through both humoral and cell-mediated immunity,and thus could Sgl"Ve as a candidate for the development of IBDV subunit vaccine. |
| Keywords: | infectious bursal disease virus (IBDV) VP2 gene IL-2 fusion protein |
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