| 通过形成类胚体诱导人羊水多能干细胞向心肌细胞分化 |
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| 引用本文： | 王晗,陈帅,程祥,窦忠英,王华岩.通过形成类胚体诱导人羊水多能干细胞向心肌细胞分化[J].生物工程学报,2008,24(9):1582-1587. |
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| 作者姓名： | 王晗 陈帅 程祥 窦忠英 王华岩 |
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| 作者单位： | 西北农林科技大学动物医学院,陕西省农业分子生物学重点实验室,陕西省干细胞工程技术研究中心,杨凌,712100 |
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| 基金项目： | 西北农林科技大学校科研和教改项目 |
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| 摘 要： | 由人羊水中分离羊水多能性干细胞,通过形成类胚体诱导其向心肌细胞分化.取人羊水标本进行体外培养,分离得到人羊水干细胞,已连续传代培养至42代,采用免疫细胞化学、RT-PCR和流式细胞仪技术对羊水干细胞的生物学特性进行检测.取10～15代羊水干细胞,悬浮培养使其形成类胚体,进而向心肌细胞诱导分化.培养的羊水干细胞呈成纤维样,表达部分胚胎干细胞特异标志基因,悬浮培养可形成类胚体.类胚体碱性磷酸酶(AP)检测呈阳性,表达三胚层特异标志基因fgf5、ζ-globin和α-fetoprotein.羊水干细胞形成类胚体后进行诱导,得到α-actin阳性细胞,表达心肌细胞特异标志基因Tbx5、Nkx2.5、GATA4和α-MHC.试验结果表明,从人羊水标本中可分离得到具有胚胎干细胞特性的细胞,经初步检测确定为羊水干细胞,并能通过形成类胚体诱导其向心肌细胞分化.
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| 关 键 词： | 人 羊水干细胞 类胚体 心肌细胞 诱导分化 |
| 收稿时间： | 2008/1/11 0:00:00 |
Differentiation of Human Amniotic Fluid Stem Cells into Cardiomyocytes through Embryonic Body Formation |
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| Han Wang,Shuai Chen,Xiang Cheng,Zhongying Dou and Huayan Wang.Differentiation of Human Amniotic Fluid Stem Cells into Cardiomyocytes through Embryonic Body Formation[J].Chinese Journal of Biotechnology,2008,24(9):1582-1587. |
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| Authors: | Han Wang Shuai Chen Xiang Cheng Zhongying Dou and Huayan Wang |
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| Institution: | Northwest A & F University, College of Veterinary Medicine, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Shaanxi Stem Cell Engineering and Technology Research Center, Yangling 712100, China;Northwest A & F University, College of Veterinary Medicine, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Shaanxi Stem Cell Engineering and Technology Research Center, Yangling 712100, China;Northwest A & F University, College of Veterinary Medicine, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Shaanxi Stem Cell Engineering and Technology Research Center, Yangling 712100, China;Northwest A & F University, College of Veterinary Medicine, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Shaanxi Stem Cell Engineering and Technology Research Center, Yangling 712100, China;Northwest A & F University, College of Veterinary Medicine, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Shaanxi Stem Cell Engineering and Technology Research Center, Yangling 712100, China |
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| Abstract: | |
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| Keywords: | human amniotic fluid stem cells embryonic body cardiomyocytes differentiation |
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