| 产紫杉醇菌株原生质体诱变育种的研究 |
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| 引用本文： | 赵凯,周东坡,平文祥,刘军,马玺,金涛.产紫杉醇菌株原生质体诱变育种的研究[J].生物工程学报,2005,21(5):848-851. |
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| 作者姓名： | 赵凯 周东坡 平文祥 刘军 马玺 金涛 |
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| 作者单位： | 1. 黑龙江大学生命科学学院,哈尔滨,150080;中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,哈尔滨,150001
2. 黑龙江大学生命科学学院,哈尔滨,150080 |
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| 基金项目： | 国家自然科学基金（No.30570025）,黑龙江省“十五”重大攻关项目（No.GA02C101）和哈尔滨市重点项目（No.011421126）资助. |
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| 摘 要： | 对紫杉醇产生菌NCEU-1的原生质体进行了紫外线和氯化锂复合诱变，筛选制霉菌素抗性突变株，共筛选出了4株正突变株。经发酵筛选试验，获得了一株遗传性状稳定、高产紫杉醇的原生质体诱变菌株——UL04-5，其紫杉醇产量从出发菌株的314.07μg/L提高至418.24μg/L。
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| 关 键 词： | 紫杉醇， 紫杉醇产生菌， 原生质体诱变 |
| 文章编号： | 1000-3061(2005)05-0848-04 |
| 收稿时间： | 05 8 2005 12:00AM |
| 修稿时间： | 07 5 2005 12:00AM |
Study on Breeding up High-yield Strain of Taxol by Protoplast Mutagensis |
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| ZHAO Kai,ZHOU Dong-Po,PING Wen-Xiang,LIU Jun,MA Xi,JIN Tao.Study on Breeding up High-yield Strain of Taxol by Protoplast Mutagensis[J].Chinese Journal of Biotechnology,2005,21(5):848-851. |
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| Authors: | ZHAO Kai ZHOU Dong-Po PING Wen-Xiang LIU Jun MA Xi JIN Tao |
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| Institution: | 1. Life and Science College of Heilongfiang Urdversity, Harbin 150080, Ch/na 2. State Key Laboratory of Veterinary Biotechrmlogy , Chinese Academy of Agricultural Sciences, Harbin 150001, China |
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| Abstract: | In order to obtain resistant mutants to nystatin, ultraviolet radiation and LiCl were used to mutagenize the protoplasts of taxol-producing fungi NCEU-1, and four positive mutants with high yield of taxol were screened out on nystatin flat. After further screening experiments on fermentation, a mutant strain--UL04-5 which was able to produce taxol with high yield and could be stably passed on in genetics was eventually found, it's ability to produce taxol was improved from 314.07 microg/L (strain NCEU-1) to 418.24 microg/L (strain U04-5). |
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| Keywords: | taxol taxol-producing fungi protoplast mutagensis |
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