| 选择性富集沙门氏菌、副溶血弧菌和霍乱弧菌的共增菌培养基SVV |
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| 引用本文： | 覃倚莹,吴晖,肖性龙,余以刚,刘冬梅,李晓凤,唐语谦.选择性富集沙门氏菌、副溶血弧菌和霍乱弧菌的共增菌培养基SVV[J].生物工程学报,2009,25(10):1497-1507. |
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| 作者姓名： | 覃倚莹 吴晖 肖性龙 余以刚 刘冬梅 李晓凤 唐语谦 |
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| 作者单位： | 华南理工大学轻工与食品学院,广州,510640 |
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| 基金项目： | 新世纪优秀人才支持计划(No.NCET-06-0746);;粤港澳关键领域重点突破项目(No.2007A0209002);;广东省教育部产学研结合项目(No.2007B090400068)资助~~ |
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| 摘 要： | 本研究通过单因素试验和响应面分析试验建立了能够选择性富集沙门氏菌、副溶血弧菌和霍乱弧菌的共增菌培养基SVV,采用平板计数法及三重荧光PCR技术验证了SVV的增菌效果。结果表明:SVV能同时富集以不同浓度比例混合的3种目标菌,37oC振荡培养18h后,菌体浓度达到105~108CFU/mL;SVV强烈抑制大部分的非目标菌;用荧光PCR方法检测经过37oC振荡培养18h的10份人工接种样品和608份实际样品,结果表明目标菌在SVV中增殖18h后菌量达到检测限以上,SVV联合荧光PCR检测方法的检出率为4.06%,比传统检测方法(3.78%)高,无假阴性和假阳性。SVV可望应用于水产品中沙门氏菌、副溶血弧菌和霍乱弧菌检测前的增菌处理,可简化检测过程,有效克服漏检,提高检出率。
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| 关 键 词： | 选择性共增菌肉汤 SVV 沙门氏菌 副溶血弧菌 霍乱弧菌 |
| 收稿时间： | 2009/6/22 0:00:00 |
A multipathogen selective enrichment broth (SVV) for simultaneous growth of Salmonella, Vibrio parahaemolyticus, and Vibrio cholerae |
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| Yiying Qin,Hui Wu,Xinglong Xiao,Yigang Yu,Dongmei Liu,Xiaofeng Li and Yuqian Tang.A multipathogen selective enrichment broth (SVV) for simultaneous growth of Salmonella, Vibrio parahaemolyticus, and Vibrio cholerae[J].Chinese Journal of Biotechnology,2009,25(10):1497-1507. |
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| Authors: | Yiying Qin Hui Wu Xinglong Xiao Yigang Yu Dongmei Liu Xiaofeng Li and Yuqian Tang |
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| Institution: | College of Light Industry & Food, South China University of Technology, Guangzhou 510640, China;College of Light Industry & Food, South China University of Technology, Guangzhou 510640, China;College of Light Industry & Food, South China University of Technology, Guangzhou 510640, China;College of Light Industry & Food, South China University of Technology, Guangzhou 510640, China;College of Light Industry & Food, South China University of Technology, Guangzhou 510640, China;College of Light Industry & Food, South China University of Technology, Guangzhou 510640, China;College of Light Industry & Food, South China University of Technology, Guangzhou 510640, China |
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| Abstract: | We formulated a selective enrichment broth (SVV) for simultaneous growth of Salmonella, Vibrio parahaemolyticus, and Vibrio cholerae by single factor experiment and response surface method. We evaluated the enrichment effect of SVV by conventional culture method and real-time PCR assay. We obtained the SVV broth by supplementting the Buffered Peptone Water (BPW) with bile salt no. 3, potassium tellurite, and sodium citrate as inhibitors, and glucose, mannitol, snhydrous sodium sulfite and sodium pyruvate as... |
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| Keywords: | multipathogen selective enrichment broth SVV Salmonella Vibrio parahaemolyticus Vibrio cholerae |
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