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药用植物栝楼的组织培养及其表达蛋白的分析
引用本文:郑树松,苑华毅,王莉江,安成才,陈章良.药用植物栝楼的组织培养及其表达蛋白的分析[J].生物工程学报,2001,17(4):420-422,T001.
作者姓名:郑树松  苑华毅  王莉江  安成才  陈章良
作者单位:1. 云南省农业科学院油料作物研究所,昆明,650205
2. 北京大学生命科学学院,蛋白质工程及植物基因工程国家重点实验室,北京,100871
基金项目:国家自然科学基金资助(39980003).
摘    要:对栝楼的快速繁殖、愈伤组织的诱导与再分化,以及不同培养体系中天花粉蛋白的表达进行了初步研究。结果表明:栝楼茎切段的腋芽和顶芽在MS+0.5、1.0mg/L 6-BA培养基上可以快速繁殖;组织培养苗的叶片切块在MS+4.0mg/L 6-BA +0.2mg/L IAA的培养基上可形成愈伤组织,该愈伤组织在30d后再分化为绿苗,绿苗分化率为0.25苗/外植体;绿苗转移至MS+0.1mg/L NAA的培养基可100%生根;生根苗移栽至土壤中100%成活;移栽成活的栝楼在30d后长出小块根,并检测到天花粉蛋白的表达。

关 键 词:栝楼,  组织培养,  天花粉蛋白
文章编号:1000-3061(2001)04-0420-04

The Tissue Culture of Medicinal Plant Trichosanthes Kirilowii and its Protein Analysis
S S Zheng,H Y Yuan,L J Wang,C C An,Z L Chen.The Tissue Culture of Medicinal Plant Trichosanthes Kirilowii and its Protein Analysis[J].Chinese Journal of Biotechnology,2001,17(4):420-422,T001.
Authors:S S Zheng  H Y Yuan  L J Wang  C C An  Z L Chen
Institution:Oil Crops Research Institute of Yunnan Academy of Agriculture Science, Kunming, 650205, China.
Abstract:We reported preliminary results of rapid propagation, callus induction and regeneration of Trichosanthes Kirilowii, and its protein analysis. Pre-existing meristerms regenerate shoots very rapidly when grown on MS medium containing 0.5 or 1.0 mg/L 6-BA; calli could be induced from leaf sections when put on MS medium containing 4.0 mg/L 6-BA + 0.2 mg/L IAA; shoots regenerated successfully 30 days after calli induction and the differentiation ratio was one shoot out of every four leaf sections; and all shoots gave rise to roots after removing onto MS medium containing 0.1 mg/L NAA and 100% survived when transplanted into soil. Very excitingly, these plants produced small tubers in one month, where satisfactory expression of TCS protein was detected by Western blot analysis.
Keywords:Trichosanthes Kirilowii Maxim  \%Tissue culture  Trichosanthin(TCS)
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