隐甲藻深层培养产生二十碳五烯酸的研究

二十碳五烯酸 (Ｃ2 0 :5 ,Ｅｉｃｏｓａｐｅｎｔａｅｎｏｉｃａｃｉｄ ,简称ＥＰＡ)是一种重要的ω 3系列人体必需多不饱和脂肪酸 (Ｐｏｌｙｕｎ ｓａｔｕｒａｔｅｄｆａｔｔｙａｃｉｄｓ,ＰＵＦＡ) 1～ 2 ] 。自从Ｄｙｅｒｂｅｒｇｅ等人报道了二十碳五烯酸对防止和治疗血栓、关节硬化、抗炎症、抗癌、促进脑组织发育等方面具有明显效果以来 ,人们对它的营养和医药价值及生产方法进行了广泛的研究3～ 6] 。目前 ,二十碳五烯酸主要来源于深海鱼油 ,但其产量不稳定 ,纯化工艺复杂且含有难以消除的鱼腥味 ,难以满足市场的需求。由于二十碳五…

Studies on Eicosapentaenoic Acid Production by Submersion Culture of Cryphecodinium cohnii

The effects of the incubation temperature, initial pH of the medium, carbon source and nitrogen source on the production of Crypthecodinium cohnii C98 were studied. Through orthogonal experiments, the optimum culture medium was obtained (g/L): Glucose, 65; Yeast extract, 2.0; KNO3 3.0, KH2PO4 1.0, MgSO4.7H2O 0.6, NaCl 5.8, CaCl2.2H2O 0.1, ZnSO4.7H2O 0.0067, FeCl3.6H2O 0.014, CuSO4.5H2O 0.0004, MnSO4.H2O 0.0002. Under the optimum culture conditions, the microalgae dry cell weight and eicosapentaenoic acid was 18.3 g/L and 0.891 g/L, respectively. The submersion culture process was analysed.