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| 水稻OsCIPK10基因的克隆和功能分析 | |
| 引用本文： | 章俊丽,杨鵾,张玉满,颜永胜,赵志强,方荣祥,孙宗修,傅亚萍,陈晓英.水稻OsCIPK10基因的克隆和功能分析[J].生物工程学报,2009,25(9):1394-1401. |
| 作者姓名： | 章俊丽 杨鵾 张玉满 颜永胜 赵志强 方荣祥 孙宗修 傅亚萍 陈晓英 |
| 作者单位： | 1. 中国科学院微生物研究所植物基因组学国家重点实验室,北京100101;中国科学院研究生院,北京100039 2. 电子科技大学生命科学与技术学院,成都,610054 3. 中国科学院微生物研究所植物基因组学国家重点实验室,北京,100101 4. 中国水稻研究所水稻生物学国家重点实验室,杭州,310006 |
| 基金项目： | 中国科学院知识创新工程项目(No.KSCXZ-YW-N-025)资助~~ |
| 摘 要： | 利用反向遗传学的方法对水稻OsCIPK10基因的功能进行了分析。结果表明,过量表达OsCIPK10基因的转基因水稻与野生型水稻在株型、抗高盐和耐低钾能力方面没有明显差异,但是小RNA干扰表达OsCIPK10基因的转基因水稻表现出显著的抗盐性。在缺钾胁迫条件下OsCIPK10基因的表达升高,推测该基因在应答高盐和低钾的非生物胁迫过程中起作用。OsCIPK10基因启动子与报告基因GUS融合表达的转基因水稻的染色结果显示:OsCIPK10基因呈组成型表达,但是在维管组织表达水平更高。 |
| 关 键 词： | 水稻 CIPK 信号转导 |
| 收稿时间： | 2009/5/11 0:00:00 |
Identification and characterization of rice OsCIPK10 gene |
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| Junli Zhang,Kun Yang,Yuman Zhang,Yongsheng Yan,Zhiqiang Zhao,Rongxiang Fang,Zongxiu Sun,Yaping Fu and Xiaoying Chen.Identification and characterization of rice OsCIPK10 gene[J].Chinese Journal of Biotechnology,2009,25(9):1394-1401. | |
| Authors: | Junli Zhang Kun Yang Yuman Zhang Yongsheng Yan Zhiqiang Zhao Rongxiang Fang Zongxiu Sun Yaping Fu and Xiaoying Chen |
| Institution: | State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Graduate University of Chinese Academy of Sciences, Beijing 100039, China;School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China;State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Graduate University of Chinese Academy of Sciences, Beijing 100039, China;State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Graduate University of Chinese Academy of Sciences, Beijing 100039, China;State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;State Key Laboratory of Rice Biology, National Rice Research Institute, Hangzhou 310006, China;State Key Laboratory of Rice Biology, National Rice Research Institute, Hangzhou 310006, China;State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China |
| Abstract: | The preliminary role of calcineurin B-like protein-interacting protein kinases(CIPKs) in stress response is defined but the exact function of OsCIPK10 gene in rice stress response and its expression pattern yet unclear.In this study we explored the possible functions of OsCIPK10 gene by reverse genetics approaches and also revealed its expression pattern by GUS staining.From the preliminary study of this gene we presumed its function to assist plant to resist stress but over-expressed OsCIPK10 rice transgen... |
| Keywords: | rice CIPK signal transduction |
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