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| 一种KBMA炭疽疫苗候选株的研制 | |
| 引用本文： | 沈非,袁盛凌,展德文,王艳春,任敏,陶好霞,王芃,王令春,陈冬生,刘纯杰.一种KBMA炭疽疫苗候选株的研制[J].生物工程学报,2011,27(5):781-789. |
| 作者姓名： | 沈非 袁盛凌 展德文 王艳春 任敏 陶好霞 王芃 王令春 陈冬生 刘纯杰 |
| 作者单位： | 1. 安徽师范大学生命科学学院,芜湖,241000;中国人民解放军军事医学科学院,生物工程研究所,病原微生物与生物安全国家重点实验室,北京,100071 2. 中国人民解放军军事医学科学院,生物工程研究所,病原微生物与生物安全国家重点实验室,北京,100071 3. 安徽师范大学生命科学学院,芜湖,241000 |
| 基金项目： | 十一五国家重大传染病专项 (No. 2008ZX10004-105) 资助。 |
| 摘 要： | 炭疽病是由炭疽芽胞杆菌Bacillus anthracis引起的一种人畜共患传染病，严重影响着人类的健康。近年来在细菌疫苗的研究中发现一种特殊的现象：细菌被杀死后，体内的代谢活性却仍然维持 (Killed but metabolically active，KBMA)。此发现为炭疽新型疫苗候选株的研制提供了新思路。先通过同源重组的方法，利用pMAD质粒和Cre-loxP重组酶系统完成对缺失两个毒性大质粒的炭疽芽胞杆菌减毒株AP422的uvrAB基因的敲除，得到AP422△uvrAB菌株，然后通过光化学处理 (包括长波紫外光的照射和8-甲氧基补骨脂素处理)，使炭疽芽胞杆菌AP422△uvrAB失去繁殖能力。利用四氮唑化合物MTS检测其代谢活性，表明光化学处理杀死后的炭疽芽胞杆菌AP422△uvrAB在至少4 h内维持一个很高的代谢活性水平，即具备典型的KBMA特性。炭疽杆菌AP422 △uvrAB的KBMA菌株的成功研制为我们提供了一种新型炭疽疫苗候选株。 |
| 关 键 词： | uvrAB基因，同源重组，Cre-LoxP系统，基因敲除，长波紫外光，8-MOP，MTS，代谢活性 |
| 收稿时间： | 2010/12/13 0:00:00 |
Development of a killed but metabolically active anthracis vaccine candidate strain |
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| Fei Shen,Shengling Yuan,Dewen Zhan,Yanchun Wang,Min Ren,Haoxia Tao,Peng Wang,Lingchun Wang,Dongsheng Chen and Chunjie Liu.Development of a killed but metabolically active anthracis vaccine candidate strain[J].Chinese Journal of Biotechnology,2011,27(5):781-789. | |
| Authors: | Fei Shen Shengling Yuan Dewen Zhan Yanchun Wang Min Ren Haoxia Tao Peng Wang Lingchun Wang Dongsheng Chen and Chunjie Liu |
| Institution: | College of Life Sciences, Anhui Normal University, Wuhu 241000, China; State Key Laboratory of Pathogen and Biosafety, Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China;State Key Laboratory of Pathogen and Biosafety, Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China;State Key Laboratory of Pathogen and Biosafety, Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China;State Key Laboratory of Pathogen and Biosafety, Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China;College of Life Sciences, Anhui Normal University, Wuhu 241000, China; State Key Laboratory of Pathogen and Biosafety, Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China;State Key Laboratory of Pathogen and Biosafety, Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China;State Key Laboratory of Pathogen and Biosafety, Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China;State Key Laboratory of Pathogen and Biosafety, Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China;College of Life Sciences, Anhui Normal University, Wuhu 241000, China;State Key Laboratory of Pathogen and Biosafety, Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China |
| Abstract: | Anthrax is a zoonosis caused by Bacillus anthracis,which seriously affects human health.In recent years,a special phenomenon is found that the metabolic active of a bacterium remains after it is killed.To development of a KBMA(killed but metabolically active) Bacillus anthracis vaccine candidate strain,a plasmid pMAD and a recombinase system Cre-loxP were used to knockout the uvrAB gene of B.anthracis AP422 which lacks both of two plasmids pXO1 and pXO2.The results of PCR and 782 ISSN1000-3061 CN11-1998/Q C... |
| Keywords: | uvrAB genes homologous recombination Cre-LoxP system gene knockout long-wave-length ultraviolet (UVA) 8-MOP MTS metabolically active |
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