外加紫杉醇对悬浮培养东北红豆杉细胞的诱导效应

研究表明外加紫杉醇能够诱导悬浮培养的东北红豆杉(Taxus cuspidata)细胞总DNA发生梯带化降解。利用mRNA差异显示技术比较了紫杉醇诱导凋亡与不诱导凋亡的东北红豆杉细胞基因表达的差异，得到了8个特异表达的cDNA克隆，经Northern杂交证实其中3个在不发生凋亡的细胞中表达，5个在凋亡的细胞中表达。对这8个cDNA克隆单向序列测定后，与GenBank/EMBL/DDBJ中同源序列进行了比较，结果表明：1个cDNA片段与拟南芥中ABA应答蛋白基因的保守区有86%的同源性；2个cDNA片段与番茄内切壳聚糖酶前体基因的保守区有50%的同源性；其他5个cDNA片段无明显的同源基因，可能是新基因。

Induced-effects by Additional Taxol in Suspension Cultures of Taxus cuspidata Cells

Apoptosis was induced by taxol treatment in suspension cultures of Taxus cuspidata cells.Differential display technique was used to investigate the induced\|gene expression between the taxol\|induced T.cuspidata cells and normal control.Eight different expressed cDNA fragments were cloned and sequenced.These differential expressed fragments were further confirmed by Northern blotting hybridization with their original total RNAs.The result showed that three of the cDNA fragments were from control RNA and five of those were from taxol\|induced T.cuspidata cells.The homology of the sequences revealed that one of the clones had 86% homology with ABA\|responsive protein gene sequence in Arabidopsis thaliana ,two of the clones had 50% homology with endochitinase precursor in tomato and the other 5 clones,which might be new gene fragments,had no significant homology with the known gene sequences in GenBank/EMBL/DDBJ.