首页 | 本学科首页   官方微博 | 高级检索  
   检索      

猪瘟甲病毒复制子载体DNA疫苗与重组腺病毒活载体疫苗联合免疫效果评价
引用本文:孙元,刘大飞,王宇飞,李娜,李宏宇,梁冰冰,仇华吉.猪瘟甲病毒复制子载体DNA疫苗与重组腺病毒活载体疫苗联合免疫效果评价[J].生物工程学报,2009,25(5):679-685.
作者姓名:孙元  刘大飞  王宇飞  李娜  李宏宇  梁冰冰  仇华吉
作者单位:中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室猪传染病研究室,哈尔滨,150001
基金项目:国家高技术研究发展计划(863计划)(No.2006AA10A204)资助~~
摘    要:本实验室先前分别将构建的猪瘟病毒E2基因重组腺病毒疫苗(rAdV-E2)和猪瘟甲病毒复制子载体DNA疫苗(pSFV1CS-E2)在猪体上进行了免疫效力评价,结果显示,rAdV-E2免疫组所有猪虽然在加强免疫后产生了比较高的猪瘟特异性中和抗体,但攻毒后个别猪表现短期体温升高和轻微病变;而pSFV1CS-E2免疫组猪只虽然在攻毒后得到了保护,但产生的抗体水平较低。为了增强猪瘟甲病毒复制子载体疫苗和猪瘟重组腺病毒活载体疫苗的免疫效果,本研究应用了复制子载体DNA疫苗初免和重组腺病毒疫苗加强免疫的初免-加强(Prime-boost)免疫策略,并在猪体上进行了评价。结果显示,所有免疫猪均产生了高水平的猪瘟特异性的中和抗体,用猪瘟强毒攻击后,pSFV1CS-E2初免组所有猪(n=5)均没有出现任何猪瘟的临床症状和病理变化,攻毒后在猪血液中也没有检测到猪瘟病毒RNA,而重组腺病毒初免组(n=5)有一头猪出现短期发热和病毒血症及轻微病理变化。这表明初免-加强免疫策略能显著提高重组疫苗的免疫效力。

关 键 词:猪瘟  甲病毒复制子载体  DNA疫苗  重组腺病毒  联合免疫  
收稿时间:2008/6/10 0:00:00

A prime-boost vaccination strategy using a Semliki Forest virus replicon vectored DNA vaccine followed by a recombinant adenovirus protects pigs from classical swine fever
Yuan Sun,Dafei Liu,Yufei Wang,Na Li,Hongyu Li,Bingbing Liang and Huaji Qiu.A prime-boost vaccination strategy using a Semliki Forest virus replicon vectored DNA vaccine followed by a recombinant adenovirus protects pigs from classical swine fever[J].Chinese Journal of Biotechnology,2009,25(5):679-685.
Authors:Yuan Sun  Dafei Liu  Yufei Wang  Na Li  Hongyu Li  Bingbing Liang and Huaji Qiu
Institution:Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China;Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China;Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China;Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China;Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China;Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China;Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
Abstract:We have previously evaluated a Semliki Forest virus(SFV) replicon vectored DNA vaccine(pSFV1CS2-E2) and a recombinant adenovirus(rAdV-E2) expressing the E2 glycoprotein of classical swine fever virus(CSFV) in pigs.The results showed that the immunized pigs were protected from virulent challenge, but few pigs showed short-term fever and occasional pathological changes following virulent challenge.To enhance the immunogenecity of the vaccines, we tried a prime-boost vaccination strategy using a combination of...
Keywords:classical swine fever virus  alphavirus replicon vector  DNA vaccine  recombinant adenovirus  prime-boost  
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《生物工程学报》浏览原始摘要信息
点击此处可从《生物工程学报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号