| 血纤蛋白粘附肽与低分子量单链尿激酶融合产物活性分析 |
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| 引用本文： | 张用书, 李秀珍, 程度胜, 刘凤云,.血纤蛋白粘附肽与低分子量单链尿激酶融合产物活性分析[J].生物工程学报,1997,13(4):415-418. |
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| 作者姓名： | 张用书 李秀珍 程度胜 刘凤云 |
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| 作者单位： | 军事医学科学院生物工程研究所; |
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| 摘 要： | 人工合成了血纤蛋白粘附肽基因，构建了粘附肽与低分子量单链尿激酶ｃＤＮＡ的融合基因，在大肠杆菌中表达了融合基因。融合基因表达产物的抗原性和天然尿激酶相同，并具有尿激酶的溶纤活性和粘附肽的抗纤维蛋白单体聚合的功能。
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| 关 键 词： | 低分子量单链尿激酶 血纤蛋白粘附肽 融合基因 |
Characterization of the Fusion Protein with a Fibrin adherent Peptide and scu PA 32K |
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| Zhang Yongshu\ Li Xiuzhen\ Cheng Dusheng\ Liu Fengyun.Characterization of the Fusion Protein with a Fibrin adherent Peptide and scu PA 32K[J].Chinese Journal of Biotechnology,1997,13(4):415-418. |
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| Authors: | Zhang Yongshu\ Li Xiuzhen\ Cheng Dusheng\ Liu Fengyun |
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| Abstract: | The fibrin adherent peptide cDNA was chemically synthesized and fused with the scu PA 32K,the single chain urokinase of low molecular weight.The fused gene was expressed in E.coli .The results of activity assays showed that the fusion product remained the same antigenicity and the fibrinolytic activity as urokinase,obtaining the ability of inhibiting fibrin monomer polymerization in vitro. |
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| Keywords: | scu PA 32K fibrin adherent peptide fusion gene |
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