| 基于基因组DNA诱变的遗传重组改造乙醇工业酵母的耐热性及发酵性能 |
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| 引用本文： | 刘秀颖,何秀萍,卢莹,张博润.基于基因组DNA诱变的遗传重组改造乙醇工业酵母的耐热性及发酵性能[J].生物工程学报,2011,27(7):1049-1056. |
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| 作者姓名： | 刘秀颖 何秀萍 卢莹 张博润 |
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| 作者单位： | 1. 中国科学院微生物研究所酵母菌分子遗传与育种实验室，北京，100101;中国科学院研究生院，北京，100049
2. 中国科学院微生物研究所酵母菌分子遗传与育种实验室，北京，100101 |
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| 基金项目： | 中国科学院知识创新重大项目 (No. KSCX1-YW-11-C4) 资助。 |
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| 摘 要： | 酵母菌是乙醇发酵工业中非常重要的微生物细胞工厂，发酵过程中温度变化胁迫一直是影响生产效率的重要瓶颈之一，选育具有广泛温度适应性的酵母菌株对提高发酵性能和降低生产成本具有重要意义。通过化学诱变和基于基因组DNA诱变的遗传重组技术对乙醇工业酵母菌的温度适应性进行改造，获得耐热性能和发酵性能得到提高的重组酿酒酵母Saccharomyces cerevisiae T44-2。重组菌株T44-2的最高生长温度比原始菌株CE6提高了3 ℃，48 ℃和52 ℃热激处理1 h，重组菌株的细胞存活率分别是原始菌株的1.84
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| 关 键 词： | 乙醇工业酵母，遗传重组，温度适应性，耐热性 |
| 收稿时间： | 2010/9/30 0:00:00 |
Improvement of thermal adaptability and fermentation of industrial ethanologenic yeast by genomic DNA mutagenesis-based genetic recombination |
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| Xiuying Liu,Xiuping He,Ying Lu and Borun Zhang.Improvement of thermal adaptability and fermentation of industrial ethanologenic yeast by genomic DNA mutagenesis-based genetic recombination[J].Chinese Journal of Biotechnology,2011,27(7):1049-1056. |
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| Authors: | Xiuying Liu Xiuping He Ying Lu and Borun Zhang |
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| Institution: | Laboratory of Yeast Molecular Genetics and Breeding, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Graduate University of Chinese Academy of Sciences, Beijing 100049, China;Laboratory of Yeast Molecular Genetics and Breeding, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;Laboratory of Yeast Molecular Genetics and Breeding, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;Laboratory of Yeast Molecular Genetics and Breeding, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | Ethanol is an attractive alternative to fossil fuels.Saccharomyces cerevisiae is the most important ethanol producer.However,in the process of industrial production of ethanol,both cell growth and fermentation of ethanologenic S.cerevisiae are dramatically affected by environmental stresses,such as thermal stress.In this study,we improved both the thermotolerance and fermentation performance of industrial ethanologenic S.cerevisiae by combined usage of chemical mutagenesis and genomic DNA mutagenesis-based ... |
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| Keywords: | ethanologenic yeast genome recombinant thermal adaptability thermotolerance |
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