| 绿巨人叶柄离体培养及植株再生 |
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| 引用本文： | 张文珠,林德钦,李梅.绿巨人叶柄离体培养及植株再生[J].亚热带植物科学,2001,30(1):32-35. |
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| 作者姓名： | 张文珠 林德钦 李梅 |
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| 作者单位： | 厦门华侨亚热带植物引种园, 福建 厦门 361002 |
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| 摘 要： | 在MS基本培养基中添加不同植物生长调节剂(mg/L)对绿巨人无菌苗的叶柄进行离体培养,结果表明,MS+6-BA 1.0+NAA 2.0+2,4-D 1.0诱导效果最好,产生愈伤组织并分化出丛芽;MS+6-BA 2.0~3.0+NAA 0.2+0.2% PVP对芽的增殖效果好;附加0.2%PVP对防止褐变有一定效果;生根培养基选用1/2MS+NAA 0.1~0.5+IBA 1.0的组合。
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| 关 键 词： | 绿巨人 叶柄 离体培养 植株再生 |
| 文章编号： | 1009-7791(2001)01-0032-04 |
| 收稿时间： | 2000-10-20 |
| 修稿时间： | 2000年10月20 |
Tissue culture and plantlet regeneration from petiole of Spathiphyllum floribundum cv. Maura Loa |
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| ZHANG Wen-zhu,LIN De-qin,LI Mei.Tissue culture and plantlet regeneration from petiole of Spathiphyllum floribundum cv. Maura Loa[J].Subtropical Plant Science,2001,30(1):32-35. |
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| Authors: | ZHANG Wen-zhu LIN De-qin LI Mei |
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| Institution: | Xiamen Overseas Chinese Subtropical Plant Introduction Garden, Xiamen 361002, Fujian China |
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| Abstract: | Petioles of Spathiphyllum floribundum cv. Maura Loa were cultured on MS medium supplemented with different concentration hormones. The results showed that MS medium containing 6-BA at 1.0mg/L, NAA at 2.0mg/L and 2,4-D at 1.0mg/L gave best results in callus induction; a combination of MS with 6-BA at 2.0~3.0mg/L, NAA at 0.2mg/L and 0.2% PVP was good for proliferation of adventitious buds; MS supplemented with 0.2% PVP could prevent from browning; and 1/2MS medium containing NAA at 0.1~0.5mg/L and IBA at 1.0mg/L was favorable for rooting. |
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| Keywords: | Spathiphyllum floribundum cv Maura Loa petiole tissue culture plantlet regeneration |
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