甘蔗花叶病毒P1基因的原核表达及其抗体制备

将扩增的甘蔗花叶病毒P1基因克隆到原核表达载体pET-32a上，转化BL21（DE3），获得重组子pET-32a-P1。超声波结果显示，所得的融合蛋白以可溶性的形式存在。SDS-PAGE检测其表达产物与目的蛋白大小一致，割胶免疫注射家免得到抗体。间接ELISA测定结果表明稀释51200倍仍呈阳性信号，并通过western blot检测，证明抗体特异性良好。

Expression and Antibody Preparation of Sugarcane Mosaic Virus P1 Protein

The target gene was subcloned into the prokaryotic expression vector pET-32a, and transformed into E.coli Bl21 (DE3), to obtain recombinant pET-32a-P1.Ultrasound results showed that the fusion protein existed in soluble form.Expression product had the same size with the target protein detected by SDS-PAGE.The antibody against the P1 protein was gained by immunized rabbit with target protein cut from SDS-PAGE.Indirect ELISA measurements proved that the antibody specificity was fine, because of that the dilution of 51200-fold not merely kept positive still, but also passed western blot.