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应用RAPD分子标记技术探讨3种石斛属植物的种间关系
引用本文:张杨,陈志宽,赖育菠,黄树林,邵红伟.应用RAPD分子标记技术探讨3种石斛属植物的种间关系[J].亚热带植物科学,2014,43(2):123-126.
作者姓名:张杨  陈志宽  赖育菠  黄树林  邵红伟
作者单位:(1.广东药学院 生命科学与生物制药学院,广东 广州 510006;2.广东省生物技术候选药物研究重点实验室, 广东 广州 510006)
基金项目:国家自然科学基金项目(31100664、31300737、81303292);广东省自然科学基金项目(S2012040007958);广东药学院师资队伍建设经费
摘    要:采用RAPD分子标记技术,分析了金钗石斛、铁皮石斛和齿瓣石斛三种石斛属植物的种间关系。10个引物产生的113条DNA扩增片段中,106条(93.81%)具有多态性,利用113个RAPD标记,计算遗传距离,利用非加权组平均法建立聚类图。结果表明,RAPD标记技术较好地从分子水平揭示金钗石斛、铁皮石斛和齿瓣石斛三种石斛属植物的遗传背景、亲缘关系,并为后期在DNA水平上对药用石斛的开发利用提供资料。

关 键 词:石斛属  RAPD分析  遗传多样性  亲缘关系  
收稿时间:2013-11-24
修稿时间:2013-11-24

Relationships among 3 Species of Dendrobium Based on RAPD Marker
ZHANG Yang,CHEN Zhi-kuan,LAI Yu-bo,HUANG Shu-lin,SHAO Hong-wei.Relationships among 3 Species of Dendrobium Based on RAPD Marker[J].Subtropical Plant Science,2014,43(2):123-126.
Authors:ZHANG Yang  CHEN Zhi-kuan  LAI Yu-bo  HUANG Shu-lin  SHAO Hong-wei
Institution:(1.College of Life Sciences and Bio-pharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong China; 2.Guangdong Provincial Key Laboratory of Biotechnology Candidate Drug Research, Guangzhou 510006, Guangdong China)
Abstract:Three species of Dendrobium, D. nobile, D. officinale and D. devonianum were analyzed using random amplified polymorphic DNA (RAPD) markers. A total of 113 bands were amplified by 10 RAPD primers, among which 106 (93.81%) bands were polymorphic. The data of 113 RAPD bands were used to generate genetic distances and to construct a dendrogram by unweighted pair group method analysis (UPGMA). The results showed that the RAPD approach was efficient in studying genetic background and relationships among these 3 species and could be used to provide abundant molecular data for the medicinal development and utilization of Dendrobium.
Keywords:Dendrobium  RAPD  genetic diversity  genetic relationship
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