| 金线莲外植体筛选及愈伤组织诱导研究 |
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| 引用本文： | 王雅英,林小华,洪璇.金线莲外植体筛选及愈伤组织诱导研究[J].亚热带植物科学,2011(3):41-43. |
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| 作者姓名： | 王雅英 林小华 洪璇 |
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| 作者单位： | 厦门医学高等专科学校厦门市中药生物工程重点实验室; |
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| 基金项目： | 厦门市重点实验室项目(No.3502Z20080012) |
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| 摘 要： | 以金线莲茎段、叶片、茎片、不定芽为试材,分别在添加6-BA、ZT、NAA、KT 5个不同处理的MS及1/2MS培养基上培养,诱导愈伤组织。结果表明,以茎段、茎片、不定芽为外植体,在MS+6-BA 2.0 mg/L+NAA0.5 mg/L、MS+NAA 2.0 mg/L+KT 0.1 mg/L和MS+6-BA 2.0 mg/L+NAA 0.5 mg/L+ZT 0.2 mg/L培养基中培养,均能成功诱导愈伤组织。不定芽为诱导愈伤组织最佳外植体,最佳培养基为MS+6-BA 2.0 mg/L+NAA 0.5 mg/L+KT 0.2 mg/L。
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| 关 键 词： | 金线莲 茎段 叶片 茎片 不定芽 愈伤组织 |
Studies on Explants Selection and Callus Induction of Anoectochilus roxburghii |
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| WANG Ya-ying,LIN Xiao-hua,HONG Xuan.Studies on Explants Selection and Callus Induction of Anoectochilus roxburghii[J].Subtropical Plant Science,2011(3):41-43. |
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| Authors: | WANG Ya-ying LIN Xiao-hua HONG Xuan |
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| Institution: | WANG Ya-ying,LIN Xiao-hua,HONG Xuan(Xiamen Medical College,Xiamen Key Laboratory of Biotechnology of Chinese Traditional Medicine,Xiamen 361008,Fujian China) |
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| Abstract: | With the stems,leaves,segments,buds of Anoectochilus roxburghiias as explants,the induction of callus was studied by culturing at 5 different culture mediums with 6-BA,ZT,NAA,KT respectively.The results showed that explants including stems,segments and buds could successfully induce callus occurrence at following culture mediums,MS + 6-BA 2.0 mg/L + NAA 0.5 mg/L,MS + NAA 2.0 mg/L + KT 0.1 mg/L and MS + 6-BA 2.0 mg/L + NAA 0.5 mg/L + ZT 0.2 mg/L.Certainly,6-BA,NAA,KT were the important factors on production ... |
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| Keywords: | Anoectochilus roxburghii stem leaves segments buds callus |
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