| 橙黄玉凤花种子萌发培养及小苗组培快繁研究 |
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| 引用本文： | 郑洲翔,刘德浩,陈智涛,廖建良.橙黄玉凤花种子萌发培养及小苗组培快繁研究[J].亚热带植物科学,2018,47(3):273-276. |
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| 作者姓名： | 郑洲翔 刘德浩 陈智涛 廖建良 |
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| 作者单位： | (1.惠州学院生命科学学院，广东 惠州 516007；2.惠州市林业科学研究所，广东 惠州 516001) |
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| 基金项目： | 广东省科技厅科技基础条件研究项目(2015A030302007)；广东省科技计划项目(2017A020213031)；惠州市科技计划项目(2016x0427042)；广东省植物学资源共享课程(粤教函2017(214)号) |
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| 摘 要： | 对橙黄玉凤花Habenaria rhodocheila种子进行无菌萌发培养以获得大量原球茎，并对原球茎组培，建立其快繁体系。结果表明，橙黄玉凤花种子以0.1%升汞消毒15 min为最佳处理。种子萌发培养基中，添加0.2%活性碳(AC)有利于种子萌发。种子萌发的最佳培养基为1/2MS＋NAA 0.5 mg·L-1＋AC 0.2%，原球茎增殖分化最佳培养基为1/2MS＋NAA 0.2 mg·L-1＋ZT 3.0 mg·L-1＋AC 0.2%；生根最佳培养基为1/2MS＋NAA 0.1 mg·L-1＋6-BA 2.0 mg·L-1＋AC 0.2%。
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| 关 键 词： | 橙黄玉凤花 组织培养 快繁 |
| 收稿时间： | 2018-07-23 |
| 修稿时间： | 2018-08-10 |
Tissue Culture and Rapid Propagation of Habenaria rhodocheila |
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| ZHENG Zhou-xiang,LIU De-hao,CHEN Zhi-tao,LIAO Jian-liang.Tissue Culture and Rapid Propagation of Habenaria rhodocheila[J].Subtropical Plant Science,2018,47(3):273-276. |
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| Authors: | ZHENG Zhou-xiang LIU De-hao CHEN Zhi-tao LIAO Jian-liang |
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| Institution: | (1.School of Life Science, Huizhou University, Huizhou 516007, Guangdong China; 2.Huizhou Institute of Forestry, Huizhou 516001, Guangdong China) |
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| Abstract: | Effects of different disinfection treatments, additives, plant growth regulators on tissue culture and rapid propagation system of Habenaria rhodocheila with seeds as explants were studied. Sterilizing the seeds with 0.1% mercuric chloride for 15 min was the best disinfection treatment. Seeds germinated only on the medium adding active carbon(AC). 0.2% active carbon in culture medium was fit for germination. The optimal medium for seed germination was 1/2MS＋NAA 0.5 mg·L-1＋AC 0.2%, the optimal medium for multiple buds formation was 1/2MS＋NAA 0.2 mg·L-1＋ZT 3.0 mg·L-1＋AC 0.2%, the optimal rooting medium was 1/2MS＋NAA 0.1 mg·L-1＋6-BA 2.0 mg·L-1＋AC 0.2%. |
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| Keywords: | Habenaria rhodocheila tissue culture rapid propagation |
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