| 蝴蝶兰原球茎增殖分化影响因子探讨 |
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| 引用本文： | 姚丽娟,徐晓薇,林绍生,游聚斌,陈中林.蝴蝶兰原球茎增殖分化影响因子探讨[J].亚热带植物科学,2004,33(3):42-44. |
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| 作者姓名： | 姚丽娟 徐晓薇 林绍生 游聚斌 陈中林 |
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| 作者单位： | 浙江省亚热带作物研究所, 浙江 温州 325005 |
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| 摘 要： | 采用3个月胚龄的种胚播种形成的原球茎,初代培养时间在2.5~3个月之间换瓶转接,原球茎生长状况较好,分化形成的芽苗生长健壮;以1/2MS为基本培养基培养30d,原球茎增殖率达332%,MS培养基更有利于分化,培养2个月分化发芽率达90.6%;附加不同细胞分裂素原球茎的增殖率为6-BA >KT >Ad+KT >Ad;NAA浓度在0~1.0mg/L,对原球茎增殖分化影响不明显。
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| 关 键 词： | 蝴蝶兰 原球茎 增殖分化 |
| 文章编号： | 1009-7791(2004)03-0042-03 |
| 收稿时间： | 2003-12-24 |
| 修稿时间： | 2003年12月24 |
Influential Factors on Multiplication and Differentiation of Phalaenopsis Protocorm-like Body(PLB) |
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| YAO Li-juan,XU Xiao-wei,LIN Shao-sheng,YOU Ju-bin,CHEN Zhong-lin.Influential Factors on Multiplication and Differentiation of Phalaenopsis Protocorm-like Body(PLB)[J].Subtropical Plant Science,2004,33(3):42-44. |
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| Authors: | YAO Li-juan XU Xiao-wei LIN Shao-sheng YOU Ju-bin CHEN Zhong-lin |
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| Institution: | The Subtropical Crop Institute of Zhejiang, Wenzhou 325005, Zhejiang China |
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| Abstract: | Rapid multiplication of PLB and inducement into young plant is the key to realized commercial production of Phalaenopsis. The first generation of PLB grew well after 2.5 to 3 months cultivation, which were formed from 3 month-old embryo. The young plant grew vigorously after differentiation. The multiplication percentage of PLB was up to 332% after 30 days cultivation on 1/2 MS medium. MS medium was advantageous to differentiation. The descending of multiplication percentage of PLB was 6-BA, KT, Ad+KT, Ad, which supplemented with different cytokinins. NAA 0~1.0mg/L gave little effect on multiplication and differentiation of PLB. |
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| Keywords: | Phalaenopsis protocorm-like body(PLB) multiplication and differentiation |
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