米曲霉6-磷酸葡萄糖脱氢酶基因gsdA的克隆及生物信息学分析

6-磷酸葡萄糖脱氢酶催化6-磷酸葡萄糖生成6-磷酸葡萄糖酸，并生成NADPH，是微生物胞内磷酸戊糖途径（PPP）的关键酶。本研究以食品安全菌米曲霉CICC2012为材料，克隆获得6-磷酸葡萄糖脱氢酶基因(GenBank登录号：JN123468)。序列分析表明，该酶是由222个氨基酸组成的亲水性蛋白；128～134位氨基酸序列DHYLGKE为活性区域；170～176位氨基酸序列GTEGRGG可能为辅因子结合位点。进化树分析表明，米曲霉6-磷酸葡萄糖脱氢酶同其他丝状真菌及酵母的G6PDH较相似。

Cloning and Bioinformatic Analysis of Glucose-6-phosphate 1-dehydrogenase Gene(gsdA) from Aspergillus oryzae

Glucose-6-phosphate 1-dehydrogenase(G6PDH) is one of the key enzymes in pentose phosphate pathway(PPP).Here,the gene encoding G6PDH is cloned from Aspergillus oryzae CICC2012.This gene was sequenced and submitted to GenBank(accession number: JN123468).The sequence was analyzed bioinformatically.The results show that G6PDH from A.oryzae is a hydrophilic enzyme consisting of 222 amino acids.The sequence from 128 to 134(DHYLGKE) is the active site,and the sequences from 170 to 176(GTEGRGG) is the possible site of coenzyme binding.The phylogenetic tree shows that G6PDH of A.oryzae is similar to other filamentous fungi and the yeast one,while distinguished from the bacterial type,the plant one,and the human one.