葶苈愈伤组织诱导及分化研究

为保护野生资源、实现人工栽培,本研究以葶苈(Draba nemorosa)嫩茎为材料,采用组织培养方法进行愈伤组织诱导与分化、不定芽生根与试管苗生根继代增殖培养,以及移栽和定植研究。结果表明,MS+6-BA0.4 mg/L+2,4-D 2.5 mg/L是愈伤组织诱导培养和继代增殖培养的理想培养基;MS+6-BA 0.6 mg/L+NAA 0.1 mg/L是愈伤组织分化培养和不定芽继代增殖培养的理想培养基;1/3MS+IAA0.6 mg/L是不定芽生根培养和生根继代增殖培养的理想培养基;试管苗移栽成活率为86.8%,定植成活率为96.4%;定植苗保持了野生葶苈的植物学性状。

The Study on Callus Inducement and Differentiation of Draba nemorosa

In order to preserve the wild resources and meet the needs of cultivation,with the method of tissue culture,tender stems of Draba nemorosa were used as material to study on callus inducement and differentiation,rooting of adventitious buds,rooting multiplication of tube seedlings,replantation and field planting.The results showed that MS+6-BA 0.4 mg/L+2,4-D 2.5 mg/L was the ideal medium for inducing callus and callus multiplication;MS+6-BA 0.6 mg/L+NAA 0.1 mg/L was suitable for callus differentiation and multiplication of adventitious buds;the best medium for rooting of adventitious buds and rooting multiplication was 1/3MS+0.6 mg/L;the transplanting survival rate of tube seedlings was 86.8% and stable planting survival rate was 96.4%;the seedlings planted in field were maintained all traits of the wild Draba nemorosa.