金樱子茎提取物体外抑菌活性研究

采用纸片扩散法和试管稀释法,研究金樱子茎不同溶剂粗提取物对临床常见病原细菌的抑菌活性。结果表明,金樱子茎水提取物对绿脓杆菌、痢疾杆菌、大肠杆菌、变形杆菌无抑菌活性;对伤寒杆菌的最低抑菌浓度(MIC)为20 mg(生药)/mL;对金黄色葡萄球菌的MIC为10 mg(生药)/mL。金樱子茎75%乙醇提取物对绿脓杆菌、伤寒杆菌、痢疾杆菌、大肠杆菌、变形杆菌无抑菌活性;对金黄色葡萄球菌MIC为10 mg(生药)/mL。金樱子茎乙酸乙酯、氯仿、石油醚提取物对绿脓杆菌、伤寒杆菌、痢疾杆菌、大肠杆菌、金黄色葡萄球菌、变形杆菌无抑菌活性。

Study on the in vitro Bacteriostatic Activity of Different Solvent Extracts from Caulis Rosae laevigatae

Caulis Rosae laevigatae was extracted with different solvents to study the bacteriostatic activity of extracts from Caulis R.laevigatae.Pseudomonas aeruginosa,Dysentery bacillus,Escherichia coli,Proteusbacillus vulgaris,Salmonella typhi,Staphylococcus aureus were used as the experimental strains.80% ethanol Coptis extracts used as comparison.The diameters of inhibition zone were determined by agar plate diffusion method.Then the minimal inhibition concentration(MIC) were observed to test dilution methods.The results show that the water solution has no bacteriostasis function to Pseudomonas aeruginosa,Dysentery bacillus,Escherichia coli,Proteusbacillus vulgaris;The MIC to Salmonella typhi is 20 mg(crude drugs)/mL;The MIC to Staphylococcus aureus is 10 mg(crude drugs)/mL.75% ethanol solution has no bacteriostasis function to Pseudomonas aeruginosa,Salmonella typhi,Dysentery bacillus,Escherichia coli,Proteusbacillus vulgaris.The MIC to Staphylococcus aureus is 10 mg(crude drugs)/mL.Ethyl acetate solution,chloroform solution,petroleum ether solution have no bacteriostasis function to all of the experimental strains.In Conclusion,the different solution of caulis R.laevigatae have indefinitely bacteriostasis function on common pathogenic bacteria.