| 不同浓度活性炭对墨兰离体培养的影响 |
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| 引用本文： | 翁锦周,林加耕,林江波.不同浓度活性炭对墨兰离体培养的影响[J].亚热带植物科学,2006,35(3):37-38,36. |
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| 作者姓名： | 翁锦周 林加耕 林江波 |
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| 作者单位： | 福建省农业科学院,闽台园艺研究中心,福建,漳州,363005 |
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| 摘 要： | 以墨兰（Cymbidium sinense）地下根状茎段为外植体，探讨不同浓度活性炭对其离体培养的影响。结果表明，在MS＋NAA2．0mg／L＋10％椰汁的培养基中加入1．0g／L活性炭对原球茎诱导效果最好：MS＋BA3.0mg／L＋NAA0.5mg／L＋4．0g／L活性炭适合于不定芽分化；在1／2MS＋NAA1．0mg／L＋BA0．5mg/L＋10％椰汁的培养基中加入0．5～3．0g/L活性炭有利于提高成苗率。
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| 关 键 词： | 墨兰 离体培养 活性炭 |
| 文章编号： | 1009-7791（2006）03-0037-02 |
| 收稿时间： | 2006-03-22 |
| 修稿时间： | 2006-03-22 |
Influence of Different Activated Carbon Concentrations on Culture in Vitro of Cymbidium sinense |
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| WENG Jin-zhou,LIN Jia-geng,LIN Jiang-bo.Influence of Different Activated Carbon Concentrations on Culture in Vitro of Cymbidium sinense[J].Subtropical Plant Science,2006,35(3):37-38,36. |
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| Authors: | WENG Jin-zhou LIN Jia-geng LIN Jiang-bo |
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| Institution: | Fujian-Taiwan Horticulture Research Center, Fujian Academy of Agricultural Sciences, Zhangzhou 363005, Fujian China |
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| Abstract: | Rhizomes were used as explants to study the influence of different activated carbon concentration on culture in vitro of Cymbidium sinense. The results indicated that the best frequency of induction of protocorms was obtained in the medium of MS containing NAA 2.0mg/L, 10% coconut milk and activated carbon 1.0g/L. The medium of MS with NAA 0.5mg/L, BA 3.0mg/L, 10% coconut and activated carbon 4.0g/L was proper for differentiation of adventitious buds, and the medium of 1/2MS with NAA 1.0mg/L, BA 0.5mg/L, 10% coconut and activated carbon 0.5-3.0g/L was suitable for planting seed. |
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| Keywords: | Cymbidium sinense culture in vitro activated carbon |
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