| 鱼腥草组织培养研究 |
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| 引用本文： | 龚伟,胡庭兴,宫渊波,张健,王景燕.鱼腥草组织培养研究[J].亚热带植物科学,2005,34(1):60-62. |
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| 作者姓名： | 龚伟 胡庭兴 宫渊波 张健 王景燕 |
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| 作者单位： | 四川农业大学,生态林业工程省级重点实验室,四川,雅安,625014 |
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| 基金项目： | 四川省森林培育重点学科建设项目资助 |
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| 摘 要： | 以鱼腥草带侧芽茎段为外植体进行组培快繁研究。结果表明,初代培养以MS 6-BA1.0mg/L NAA0.05mg/L 为最佳,30d 侧芽诱导率可达66.7%;MS 6-BA 2.0 mg/L 2,4-D 0.2mg/L NAA 0.2mg/L 对芽苗的继代增殖效果最好,40d 芽苗的增殖倍数可达7.4,且芽苗较高;以MS 6-BA3.0mg/L 2,4-D 0.5mg/L NAA0.1mg/L 对芽苗的继代增重效果最好,40d 芽苗鲜重为7.358g;以沙和蛭石(1﹕1)为基质瓶外生根效果最好,30d 芽苗生根率可达84%。
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| 关 键 词： | 鱼腥草 侧芽培养 生物量 瓶外生根 |
| 文章编号： | 1009-7791(2005)01-0060-03 |
| 修稿时间： | 2004年9月2日 |
Tissue Culture of Houttuynia cordata |
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| GONG Wei,HU Ting-xing,GONG Yuan-bo,ZHANG Jian,WANG Jing-yan.Tissue Culture of Houttuynia cordata[J].Subtropical Plant Science,2005,34(1):60-62. |
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| Authors: | GONG Wei HU Ting-xing GONG Yuan-bo ZHANG Jian WANG Jing-yan |
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| Abstract: | Taking stem segments with lateral buds as explants, the tissue culture of Houttuynia cordata were studied. The results showed that MS 6-BA 1.0mg/L NAA 0.05mg/L was best for bud induction and the induction rate could reach 66.7% when cultured for 30d; MS 6-BA 2.0mg/L 2,4-D 0.2mg/L NAA 0.2mg/L gave the best result for subculture of shoots and the multiplication rate could reach 7.4 times in 40d; MS 6-BA 3.0mg/L 2,4-D 0.5mg/L NAA 0.1mg/L was best for increasing the weight of shoots and the fresh weight of shoots gain 7.358g in 40d; sand and vermiculite(1:1) was suitable for ex vitro shoots rooting and the rooting rate could reach 84 %. |
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| Keywords: | Houttuynia cordata lateral bud culture biomass ex vitro rooting |
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