| 乙二醇对木瓜蛋白酶催化活性的影响 |
| |
| 引用本文： | 初志战,巫光宏,黄卓烈.乙二醇对木瓜蛋白酶催化活性的影响[J].亚热带植物科学,2014(1):1-3. |
| |
| 作者姓名： | 初志战 巫光宏 黄卓烈 |
| |
| 作者单位： | 华南农业大学 生命科学学院、广东省农业生物蛋白质功能与调控重点实验室,广东广州510642 |
| |
| 基金项目： | 国家自然科学基金（No.10074016、No.10174021）资助 |
| |
| 摘 要： | 对不同浓度乙二醇水溶液影响木瓜蛋白酶水解酪蛋白的催化活性及构象的改变进行研究。结果表明,木瓜蛋白酶在乙二醇水溶液中水解酪蛋白的活性显著下降。动力学测定表明,木瓜蛋白酶在乙二醇水溶液中最大反应速度明显下降;差示光谱显示,在乙二醇水溶液中木瓜蛋白酶的二级结构发生了变化;荧光发射光谱显示,木瓜蛋白酶在乙二醇水溶液中发射峰位几乎没移动,但发射强度明显增高。
|
| 关 键 词： | 木瓜蛋白酶 乙二醇 酶活性 动力学 构象 |
Effect of Ethanol on Catalytic Activity of Papain |
| |
| CHU Zhi-zhan,WU Guang-hong,HUANG Zhuo-lie.Effect of Ethanol on Catalytic Activity of Papain[J].Subtropical Plant Science,2014(1):1-3. |
| |
| Authors: | CHU Zhi-zhan WU Guang-hong HUANG Zhuo-lie |
| |
| Institution: | (College of Life Science, South China Agriculture University; Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, Guangzhou 510642, Guangdong China) |
| |
| Abstract: | The alterations of papain activity and conformation were studied in ethylene glycol solutions at different concentrations. The result showed that the papain activity was obviously decreased and the V max value was declined when treated with ethylene glycol. Ultraviolet difference spectra showed that the secondary structure of papain was altered in ethylene glycol solutions. Fluorescence emission spectra showed that the peak of fluorescence emission was not shifted, but the fluorescence intensity of papain increased in ethylene glycol solutions. |
| |
| Keywords: | papain ethylene glycol enzyme activity kinetics conformation |
| 本文献已被 CNKI 维普 等数据库收录！ |
