4个火焰兰属内及属间杂交新组合F_1代核型分析

采用染色体常规压片法对兰科Orchidaceae火焰兰属Renanthera内3个及与万代兰属Vanda间1个共4个杂交新组合F1代核型进行研究。结果表明:4个杂交新组合F1代中,麒麟火焰兰R.coccinea‘Qi Lin’、麒麟火焰兰×中华火焰兰R.sinica、(云南火焰兰R.imschootiana×中华火焰兰)×麒麟火焰兰、云南火焰兰×小花万代兰Vanda coerulescens的染色体数目依次为37、57、54、38;核型公式分别为:2n=2x+1=37=22m(2sat)+15sm(4sat)、2n=3x=57=39m(3sat)+12sm(3sat)+3st+3T、2n=3x=54=9M+27m+12sm+3st+3T、2n=2x=38=6M+14m+16sm+2T。核型不对称系数依次为:48.76、60.50、51.60、65.87。按Stebbins分类,均为"2B"型。4个新组合中,染色体存在非整倍体(三体)及多倍体(3×)现象。

Karyotype Analysis of Four Intragenus and Intergenus Hybridized Combinations F 1 Generation of Renanthera

The chromosome number and karyotype of four hybridized combinations F1 generation including three intragenus combinations of Renanthera and one intergenus combination of Renanthera with Vanda were studied with normal squashing method in this paper. The results showed that the chromosome number of R. coccinea ‘Qi Lin’, R. coccinea ‘Qi Lin’ × R. sinica, （R. imschootiana×R. sinica）×R. coccinea‘Qi Lin’ and R. imschootiana×Vanda coerulescens was 37, 57, 54, 38, and the karyotypes of these were formulated as 2n=2x＋1=37=22m（2sat）＋15sm（4sat）, 2n=3x=57=39m（3sat）＋12sm（3sat）＋3st＋3T, 2n=3x=54=9M＋27m＋12sm＋3st＋3T, 2n=2x=38=6M＋14m＋16sm＋2T in turn. Asysmmetry coefficient of nuclear is 48.76, 60.50, 51.60, 65.87 respectively. The karyotypes of all examined belonged to 2B type of Stebbins. Aneuploid and polyploid were found in these hybridized combinations. The karyotypes of four F 1 generations were richer in variation.