| 不同培养条件对光叶楮试管苗玻璃化的影响 |
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| 引用本文： | 时群,韦大器.不同培养条件对光叶楮试管苗玻璃化的影响[J].亚热带植物科学,2007,36(3):12-14. |
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| 作者姓名： | 时群 韦大器 |
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| 作者单位： | 1. 钦州市林业科学研究所, 广西 钦州 535000;
2. 湖南农业大学 作物基因工程湖南省重点实验室, 湖南 长沙 410128 |
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| 基金项目： | 广西壮族自治区林业局林业专项补助项目 |
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| 摘 要： | 以MS为基本培养基,研究生长调节剂、蔗糖、琼脂、光照强度和温度等因素对光叶楮试管苗玻璃化的影响。结果表明,最佳的生长调节剂配比是1.0 mg/L 6-BA、0.3 mg/L NAA,蔗糖浓度3.5%,琼脂浓度0.35%,光照强度2 000 lx,培养温度25℃。该优化培养条件能有效防止玻璃化苗的发生,获得较高的增殖系数和较健壮的苗木,且能降低生产成本。
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| 关 键 词： | 光叶楮 培养条件 玻璃化 |
| 文章编号： | 1009-7791（2007）03-0012-03 |
| 收稿时间： | 2007-04-16 |
| 修稿时间： | 2007-04-16 |
Effects of Different Conditions of Tissue Culture on Vitrification of Plantlets from Broussonetia papyrifera |
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| SHI Qun,WEI Da-qi.Effects of Different Conditions of Tissue Culture on Vitrification of Plantlets from Broussonetia papyrifera[J].Subtropical Plant Science,2007,36(3):12-14. |
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| Authors: | SHI Qun WEI Da-qi |
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| Institution: | 1. Qinzhou Forestry Research Institute, Qinzhou 535000, Guangxi China;
2. Crop Gene Engineering Key Laboratory of Hunan Province, Hunan Agricultural University, Changsha 410128, Hunan China |
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| Abstract: | MS medium was chosen as the basic medium and the main factors such as hormone, sucrose, agar, culture irradiation and temperature were investigated on vitrification of plantlets from Broussonetia papyrifera.The results showed that the coefficient of proliferation was higher and the rate of vitrification was lower when the plantlets from B. papyrifera were cultured in MS medium supplemented with 1.0 mg/L 6-BA, 0.3 mg/L NAA, 3.5% sucrose, 0.35% agar and under the conditions of culture illumination 2 000 lx and temperature 25℃. |
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| Keywords: | Broussonetia papyrifera conditions of tissue culture vitrification |
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