Influence of different inhibitors on the activity of theRAD54 dependent step of DNA repair inSaccharomyces cerevisiae |
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Authors: | W Siede Sabine Obermaier Friederike Eckardt |
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Institution: | (1) Abteilung für Strahlenbiologie, Gesellschaft für Strahlen- und Umweltforschung, Ingolstädter Landstrasse 1, D-8042 Neuherberg, Germany |
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Abstract: | Summary The recombinagenic pathway of DNA repair in yeast was characterized by the effect of different inhibitors on the temperature-dependent survival after-irradiation in haploid cells of the thermoconditional mutantrad54-3. Blocking protein synthesis with cycloheximide in replicating cells caused partial inhibiton of theRAD54 dependent function but some repair activity remained detectable. This indicates that-rays can induceRAD54 activity above some constitutive level of function. Inhibition of DNA replication by hydroxyurea efficiently blocked theRAD54 dependent function in stationary-phase cells but not in logarithmic-phase cells. In logarithmic-phase cells, we found a strong inhibitory effect of caffeine on theRAD54 mediated repair process. |
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