Differentiation in vitro of larval and adult muscles from embryonic cells ofDrosophila |
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Authors: | Andreas Dübendorfer Annemarie Blumer Ilan Ivan Deàk |
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Institution: | (1) Zoologisches Institut der Universität Zürich, Künstlergasse 16, 8006 Zürich, Switzerland |
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Abstract: | Summary The differentiation of muscles in primary cultures of cells fromDrosophila melanogaster embryos was investigated. In early cultures, and in the absence of exogenous ecdysone, two main classes of muscle were found. Comparison, by light and electron microscopy, of one of these classes (the myotube class) with muscles from third instar larvae shows that this class corresponds to the muscles of the body wall of the larva. When - or -ecdysone is added to the cultures, these undergo a number of metamorphic changes. Most of the larval muscles disappear, and two new types of muscle form. Ultrastructural and light microscopic examination of these two types indicates that they correspond to the two classes of skeletal muscle (fibrillar and tubular) found in adult flies. |
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Keywords: | Tissue culture Muscles Metamorphosis Ecdysone Drosophila |
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