Identification and Genetic Analysis of a Novel Allelic Variation of <i>Brittle-1</i> with Endosperm Mutant in Maize |
| |
Authors: | Sen Wang Fei Zheng Meijing Zhang Jun Tu Yanping Chen Jianhua Yuan Qingchang Meng |
| |
Institution: | 1 Institute of Food Crops, Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China2 Jiangsu Zhongjiang Seed Co., Ltd., Nanjing, 211500, China3 Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, 210095, China |
| |
Abstract: | Endosperm mutants are critical to the studies on both starch synthesis
and metabolism and genetic improvement of starch quality in maize. In the present study, a novel maize endosperm mutant A0178 of natural variation was used
as the experimental material and identified and then characterized. Through phenotypic identification, genetic analysis, main ingredients measurement and
embryo rescue, development of genetic mapping population from A0178, the
endosperm mutant gene was located. The results showed that the mutant exhibited
extremely low germination ability as attributed to the inhibited embryo development, and amounts of sugars were accumulated in the mutant seeds and more
sugars content was detected at 23 days after pollination (DAP) in A0178 than
B73. Employing genetic linkage analysis, the mutant trait was mapped in the
bin 5.04 on chromosome 5. Sequence analysis showed that two sites of base transversion and insertion presented in the protein coding region and non-coding
region of the mutant brittle-1 (bt1), the adenylate translocator encoding gene
involved in the starch synthesis. The single base insertion in the coding region
cause frameshift mutation, early termination and lose of function of Brittle-1
(BT1). All results suggested that bt1 is a novel allelic gene and the causal gene
of this endosperm mutant, providing insights on the mechanism of endosperm formation in maize. |
| |
Keywords: | Maize endosperm mutant Brittle-1" target="_blank">Brittle-1" target="_blank">Brittle-1 gene mapping allelic variation |
|
| 点击此处可从《Phyton》浏览原始摘要信息 |
| 点击此处可从《Phyton》下载免费的PDF全文 |
|