Proteome response of Escherichia coli fed-batch culture to temperature downshift |
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Authors: | Yang-Hoon Kim Kyung Yeon Han Kibeom Lee Jeewon Lee |
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Institution: | (1) Department of Chemical and Biological Engineering, Korea University, Anam-Dong 5-1, Sungbuk-Ku, Seoul, 136-713, South Korea;(2) The Wistar Institute, Philadelphia, PA, USA |
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Abstract: | During fed-batch cultivation of Escherichia coli K-12, the proteomic response to a temperature downshift from 37 to 20°C was quantitatively monitored and analyzed by using
two-dimensional electrophoresis. When the temperature of exponentially growing E. coli K-12 culture was downshifted to 20°C, the synthesis level of 57 intracellular proteins showed significant changes for a prolonged
period of time, compared to the fed-batch culture controlled at 37°C. Thus, these proteins are regarded as important stress
proteins responsive to cold shock, which were analyzed by using matrix-assisted laser desorption/ionization time-of-flight
mass spectrometry and identified using the E. coli SWISS-2DPAGE database. Most of the identified proteins were shown to be involved in energy metabolism, several cellular molecule
biosynthetic pathways and catabolism, cell processes, flagellar biosynthesis and motility, and protein translation and folding.
The systematic approach to the monitoring of proteomic responses and the detailed analysis results reported in this article
would be useful in understanding the metabolic adaptation to lowered culture temperature and designing efficient fermentation
strategies for the production of recombinant proteins and metabolites using E. coli strains. |
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