Biosynthesis of citric acid by Yarrowia lipolytica repeat-batch culture on ethanol |
| |
| Authors: | T E Arzumanov N V Shishkanova T V Finogenova |
| |
| Institution: | (1) G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences, Moscow region, Russia, RU;(2) IBPM, p-t Nauki 5, Pushchino, Moscow region, 142290 Russia e-mail: finog@ibpm.serpukhov.su Tel.: +7-0967-730990 Fax: +7-095-923-3602 , RU |
| |
| Abstract: | After analysis of batch culture and identification of the ways for prolongation of citric acid active synthesis by yeast,
repeat-batch (RB) cultivation was suggested. Yarrowia lipolytica strain RB cultivation was studied and optimal conditions for cultivation selected. It was shown that when applying RB cultivation,
better results were obtained than for batch cultivation. The activity of the culture remained stable after cultivation for
more than 700 h. Comparative analysis of enzyme activities confirmed the regularity of the effect described, as the activity
of practically of all the enzymes participating in ethanol oxidation and citric acid biosynthesis remained stable over time
during RB cultivation. Advantages of RB cultivation for the production of citric acid by yeast are discussed.
Received: 1 March 1999 / Received revision: 28 June 1999 / Accepted: 5 July 1999 |
| |
| Keywords: | |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
