Production of exopolysaccharide by Pseudomonas sp. ATCC 31461 (Pseudomonas elodea ) using whey as fermentation substrate |
| |
Authors: | A M Dlamini P S Peiris |
| |
Institution: | (1) School of Applied and Environmental Science, University of Western Sydney–Hawkesbury, Bourke Street, Richmond, NSW 2753, Australia. Fax: 61 2 045 701 267, AU |
| |
Abstract: | An improved strain of Pseudomonas sp. ATCC 31461 (Pseudomonas elodea), capable of producing broth viscosities of 11 000 and 4700 mPa s (cP) when grown in enriched whey permeate and enriched
sweet whey broths respectively, was isolated. The isolation was by serial transfers of the parent on lactose-rich and sweet
whey broths. Maximum viscosities and biopolymer production were observed in 25% (v/v) whey concentration. In whey concentrations
of 50% (v/v) or greater, residual glucose was detected in the broth and biopolymer production was low. This strain is capable
of totally utilising the lactose in up to 50% (v/v) whey in 64 h. Enzyme activities suggest that the transport of lactose
in P. elodea is by the permease system as opposed to the phosphotransferase system. The location of β-galactosidase is mainly intracellular.
The improved strain is able to utilise lactose better than the parent and produce 1.6 times more intracellular β-galactosidase
activity compared to the parent.
Received: 3 May 1996 / Received revision: 8 August 1996 / Accepted: 10 August 1996 |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|