Activation of an indigenous microbial consortium for bioaugmentation of pentachlorophenol/creosate contaminated soils |
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Authors: | Marie-Paule Otte Josée Gagnon Yves Comeau Nathalie Matte Charles W Greer Réjean Smason |
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Institution: | (1) Environmental Sciences Institute, University of Quebec in Montreal, Station A, P.O. Box 88 88, H3C 3P8 Montreal, Quebec, Canada;(2) Environmental Engineering Section, Department of Civil Engineering, Ecole Polytechnique of Montreal, Station A, P.O. Box 60 79, H3C 3A7 Montreal, Quebec, Canada;(3) Environmental Engineering Group, Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Avenue, H4P 2R2 Montreal, Quebec, Canada |
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Abstract: | Soil activation, a concept based on the cultivation of biomass from a fraction of a comtaminated soil for subsequent use as an inoculum for bioaugmentation of the same soil, was studied as a method for the aerobic biodegradation of pentachlorophenol (PCP) and polycyclic hydrocarbons (PAH) in contaminated soils. A microbial consortium able to degrade PCP and PAH in contaminated soil from wood-preserving facilities was isolated and characterized for PCP degradation and resistance. To obtain an active consortium from the contaminated soil in a fed-batch bioreactor, the presence of soil as a support or source of nutrients was found to be essential. During the 35 days of bioreactor operation, residual PCP in solution remained near zero up to a loading rate of 700mg/l per day. The PCP meneralization rate increased from 70 mg/l per day when no PCP was added to the bioreactor to 700 mg/l per day at the maximum loading rate. The consortium tolerated a PCP concentration of 400 mg/l in batch experiments. Production of a PCP-degrading consortium in a fed-batch slurry bioreactor enhanced the activity of PCP biodegradation by a factor of ten. PAH biodegradation increased, during the same time period, by a factor of 30 and 81 for phenanthrene and pyrene, respectively. Preliminary laboratory-scale results indicated that a significant reduction in the time required for degradation of PCP and PAH in contaminated soil could be achieved using activated soil as an inoculum.Issued as NRC 33861
correspondence to: R. Samson |
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