Formate production from methanol by formaldehyde dismutase coupled with a methanol oxidation system |
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Authors: | Nobuo Kato Sumiko Mizuno Yukio Imada Masayuki Shimao Chikahiro Sakazawa |
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Institution: | (1) Department of Environmental Chemistry and Technology, Tottori University, 680 Tottori, Japan;(2) Research Center, Mitsubishi Chemical Industries, Kamoshida-cho, Midori-ku, 227 Yokohama, Japan |
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Abstract: | Summary Formaldehyde dismutase was greatly stabilized by immobilization in a urethane prepolymer (PU-6). The immobilized enzyme exhibited stochiometrical dismutation of formaldehyde to methanol and formate in several repeated reactions. Conversion of methanol to formate occurred in a reaction with an immobilized enzyme system consisting of alcohol oxidase, catalase and formaldehyde dismutase, and with an intact cell-mixture of Hansenula polymorpha and Pseudomonas putida. Furthermore, the stability of the cell-mixture during repeated reactions was greatly improved by the immobilization, the 600 mM methanol added periodically being converted to formate in a 75% yield in 12 h. The immobilized cellsystem was also effective for the conversion of several aliphatic alcohols, C1 to C4, to the corresponding acids. |
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