Peptide diversity in strains of the cyanobacterium <Emphasis Type="Italic">Microcystis aeruginosa</Emphasis> isolated from Portuguese water supplies |
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Authors: | Joana Martins Martin L Saker Cristiana Moreira Martin Welker Jutta Fastner Vitor M Vasconcelos |
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Institution: | 1.Centro Interdisciplinar de Investiga??o Marinha e Ambiental, CIIMAR/CIMAR–LA,Porto,Portugal;2.Institut für Chemie, AG Biochemie und Molekulare Biologie,Technische Universit?t Berlin,Berlin,Germany;3.Departamento de Zoologia e Antropologia, Faculdade de Ciências,Universidade do Porto,Porto,Portugal |
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Abstract: | Strains of the cyanobacterium Microcystis aeruginosa were isolated into pure culture from a variety of lakes, rivers, and reservoirs in Portugal. Samples were tested with matrix-assisted
laser desorption/ionization–time-of-flight mass spectrometry (MALDI-TOF MS) to investigate the presence of various peptide
groups including aeruginosins, microginins, anabaenopeptins, cyanopeptilins, microcystins, and microviridins and other peptide-like
compounds. Binary data, based on the presence and absence of different peptide groups, were analyzed by phylogenetic inference.
DNA was also extracted from the samples and tested using a range of primers. Those strains that gave positive results for
a Microcystis-specific primer pair were further analyzed for the presence of genes linked to the biosynthesis of microginin and microcystin.
The results showed that a wide range of microcystin forms were produced by the strains among which MC-LR, -FR, -RR, -WR, and
-YR were the most common. The peptide profiles obtained from the MALDI analysis were assessed using cluster analysis which
resulted in the formation of distinct groups or chemotypes. |
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Keywords: | Microcystis Peptides |
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