Immobilization of photosynthetically active cyanobacteria in glutaraldehyde-crosslinked albumin matrix |
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Authors: | G C Papageorgiou T Lagoyanni |
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Institution: | (1) National Research Center Demokritos, Department of Biology, Aghia Paraskevi, 15310 Athens, Greece |
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Abstract: | Summary Cryoprotection was afforded to cells ofPhormidium luridum andAnacystis nidulans by preloading them with dimethyl sulphoxide and then performing the immobilization reaction in dimethyl sulphoxide-free medium.
Compared with free cyanobacteria cells, matrix-immobilized cyanobacteria exhibit superior temperature tolerance and storage
longevity of photoinduced electron transport, while their phycobiliproteins are better protected against thermal denaturation.
Photosynthetically activePhormidium spheroplasts capable of interacting with ionic Hill cofactors, were immobilized in crosslinked albumin matrix, with cryoprotection
provided by sorbitol. |
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