d-Xylose as inducer of the xylan-degrading enzyme system in the yeast Pullularia pullulans |
| |
Authors: | Juana Pou-Llinas Hugues Driguez |
| |
Institution: | (1) Centre de Recherches sur les Macromolécules Végétales, CNRS-Grenoble, B.P. 68, F-38402 Saint Martin d'Hères, France;(2) Present address: Instituto de Fermentaciones Industriales, C.S.I.C., Juan de la Cierva 3, Madrid 6, Spain |
| |
Abstract: | Summary The specificity of induction of wooddegrading enzymes from Pullularia pullulans was investigated using series of mono-, di- and (14)--trisaccharides or glycanes. A strain of P. pullulans (1740), unable to grow on Avicel or carboxymethyl-cellulose (CMC), uses xylan and steamexploded wood as carbon sources. This strain, thus grown, was evaluated for various enzyme activities. d-Xylose was the nutritional inducer of -xylosidase and -xylanase. d-Glucuronic acid induced activity on CMC and -glucosidase activity was observed regardless of carbon source used. (14)--Xylobiose was not an inducer of -xylanase production, but high levels of this enzyme were obtained with either structural isomers (12) or (13)-. Since synthesis of this enzyme was stimulated by increasing xylose concentration yp to 40 g/l, it is suggested that xylose enters the cells by passive transport and is unable to induce a permease system.Affiliated to the Scientific, Technological and Medical University of Grenoble |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|