Ceramic honeycomb as support for covalent immobilization of laccase from Trametes versicolor and transformation of nuclear fast red |
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Authors: | Plagemann Regina Jonas Ludwig Kragl Udo |
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Institution: | (1) Institute of Chemistry, University of Rostock, Albert-Einstein-Strasse 3a, 18059 Rostock, Germany;(2) Electron Microscopic Center, Medical Faculty Institute of Medicine, University of Rostock, Strempelstrasse 14, 18055 Rostock, Germany; |
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Abstract: | The covalent immobilization of laccase on an inorganic ceramic support was investigated. The intention was to find a system
of enzyme and reactor for a universal immobilization procedure. Laccase from Trametes versicolor as model enzyme was chosen. The special honeycomb structure of the monolith can be applied for intensive mixing of the reaction
compounds. An appropriate reactor with ceramic material was constructed allowing different setup for enzyme immobilization
and its application. To test the success of the immobilization, 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) was
used. The immobilized laccase was found to be stable over a time period of over 3 months. As an example for possible application
for treatment of wastewater containing dyes, the conversion of nuclear fast red as model substrate was tested. |
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