Optimizing emulsan production of <Emphasis Type="Italic">A. venetianus</Emphasis> RAG-1 using response surface methodology |
| |
Authors: | Wen-Ta Su Wen-Jang Chen Yi-Fan Lin |
| |
Institution: | (1) Department of Chemical Engineering, National Taipei University of Technology, 1 Sec. 3, Chung-Hsiao E. Rd, Taipei, 10608, Taiwan |
| |
Abstract: | Statistical experimental design was used to optimize medium constituents for emulsan production by Acinetobacter venetianus RAG-1 in batch cultivation. The factors affecting emulsan production were screened by a two-level factorial design, and the
optimal concentration of medium constituents for emulsan production were determined by the method of steepest path ascent
and central composite experimental design. Experimental results showed that the optimal medium constituents were 9.16 g/L
ethanol, 8.2 g/L KH2PO4, 23.32 g/L K2HPO4, 5.77 g/L (NH4)2SO4 and 0.354 g/L MgSO4•7H2O. Under this optimal composition, the predicted emulsan production was 72.198 mg/L, and experimental value was 73.312 mg/L
for 80 h culture in the shake flasks, and the emulsan yield by A. venetianus RAG-1 was enhanced nearly 1.48-fold (from 49.5 to 73.312 mg/L). Based on the results, we identify the optimal medium constituents
for emulsan production and could take advantage of strategy for scale up the fermentation of emulsan production. |
| |
Keywords: | Optimization Emulsan Acinetobacter venetianus RAG-1 Ethanol Response surface methodology |
本文献已被 SpringerLink 等数据库收录! |
|