Comparison of partial 16S rRNA gene sequences obtained from activated sludge bacteria |
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Authors: | E J Vainio A Moilanen T T Koivula D H Bamford J Hantula |
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Institution: | (1) Forest Research Institute, P.O. Box 18, FIN-01301 Vantaa, Finland Tel.: +358 9 8570 5682 Fax: +358 9 8572 575 e-mail: jarkko.hantula@metla.fi, FI;(2) Department of Ecology and Systematics, Division of Population Biology, P.O. Box 17, FIN-00014 University of Helsinki, Finland, FI;(3) Department of Biosciences, Division of Microbiology, P.O. Box 41, FIN-00014 University of Helsinki, Finland, FI;(4) Institute of Biotechnology and Department of Biosciences, Division of Genetics, P.O. Box 56, FIN-00014 University of Helsinki, Finland, FI |
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Abstract: | The cultivated and uncultivated bacterial communities of an activated sludge plant were studied. Two samples were taken and
a total of 516 bacterial isolates were classified into groups using their whole-cell protein patterns. The distribution of
bacteria into protein-pattern groups differed significantly between the two samples, suggesting variation in culturable bacterial
flora. Partial 16S rRNA gene sequences were determined for representatives of the commonest protein-pattern groups. Most of
the sequences obtained were previously unknown, but relatively closely related to known sequences of organisms belonging to
the α, β or γ subclasses of the proteobacteria, the first two subclasses being predominant. This classification of bacteria
isolated on a diluted nutrient-rich medium differed from recent culture-dependent studies using nutrient-rich media. The uncultivated
bacterial community was studied by analyzing ten partial 16S rRNA gene sequences cloned directly from activated sludge. None
of the cloned sequences was identical to those determined for culturable organisms; or to those in the GenBank database. They
were, however, related to the α or β subclasses of the proteobacteria, or to the gram-positive bacteria with a high G+C DNA
content.
Received: 4 November 1996 / Received revision: 24 February 1997 / Accepted: 28 February 1997 |
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