Prokaryotic expression of a constitutively expressed <Emphasis Type="Italic">Tephrosia villosa</Emphasis> defensin and its potent antifungal activity |
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Authors: | S Vijayan Lalitha Guruprasad P B Kirti |
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Institution: | (1) Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046, India;(2) School of Chemistry, University of Hyderabad, Hyderabad, 500046, India |
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Abstract: | Plant defensins are small, highly stable, cysteine-rich antimicrobial peptides produced by the plants for inhibiting a broad-spectrum
of microbial pathogens. Some of the well-characterized plant defensins exhibit potent antifungal activity on certain pathogenic
fungal species only. We characterized a defensin, TvD1 from a weedy leguminous herb, Tephrosia villosa. The open reading frame of the cDNA was 228 bp, which codes for a peptide with 75 amino acids. Expression analyses indicated
that this defensin is expressed constitutively in T. villosa with leaf, stem, root, and seed showing almost similar levels of high expression. The recombinant peptide (rTvD1), expressed
in the Escherichia coli expression system, exhibited potent in vitro antifungal activity against several filamentous soil-borne fungal pathogens.
The purified peptide also showed significant inhibition of root elongation in Arabidopsis seedlings, subsequently affecting the extension of growing root hairs indicating that it has the potential to disturb the
plant growth and development. |
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Keywords: | Tephrosia villosa TvD1 Constitutive expression Prokaryotic expression Antifungal activity Arabidopsis root growth inhibition |
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