Transformation of lithocholic acid to a new bile acid, 3α, 15β-dihydroxy-5β-cholanic acid by Cunninghamella blakesleeana ST-22 |
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| Authors: | Songsri Kulprecha Takuya Nihira Kazufumi Yamada Toshiomi Yoshida Naline Nilubol Hisaharu Taguchi |
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| Institution: | (1) Faculty of Engineering, International Center of Cooperative Research and Development in Microbial Engineering, Osaka University, 2-1 Yamada-oka, 565 Suita-shi, Osaka, Japan;(2) Present address: Department of Microbiology, Faculty of Science, Chulalongkorn University, 10 500 Bangkok, Thailand |
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| Abstract: | Summary A fungus identified as Cunninghamella blakesleeana (Lendner) can carry out 15 -hydroxylation of lithocholic acid to a new bile acid (3 ,15-dihydroxy-5-cholanic acid). By optimizing the fermentation conditions, the amount of the product increased from 0.17 g/l to 1.2 g/l. Hydrophilicity measurements and in vitro cholesterol solubilization tests showed that 3, 15-dihydroxy-5-cholanic acid was as effective as ursodeoxycholic acid in cholesterol solubilization.Abbreviations LCA
lithocholic acid (3-hydroxy-5-cholanic acid)
- 3, 15-DHC
(3, 15-dihydroxy-5-cholanic acid)
- DMSO
dimethyl sulfoxide
- CHES
2-N-cyclohexylamino]ethanesulfonic acid |
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