Expression and export: recombinant protein production systems for Aspergillus |
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Authors: | André Fleißner Petra Dersch |
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Institution: | 1. Institute of Genetics, Technische Universit?t Braunschweig, Spielmannstrasse 7, 38106, Braunschweig, Germany 2. Department of Molecular Infection Biology, Helmholtz Centre for Infection Research, Inhoffenstrasse. 7, 38124, Braunschweig, Germany 3. Helmholtz-Zentrum für Infektionsforschung, Inhoffenstrasse 7, 38124, Braunschweig, Germany
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Abstract: | Several Aspergillus species, in particular Aspergillus niger and Aspergillus oryzae, are widely used as protein production hosts in various biotechnological applications. In order to improve the expression
and secretion of recombinant proteins in these filamentous fungi, several novel genetic engineering strategies have been developed
in recent years. This review describes state-of-the-art genetic manipulation technologies used for strain improvement, as
well as recent advances in designing the most appropriate engineering strategy for a particular protein production process.
Furthermore, current developments in identifying bottlenecks in the protein production and secretion pathways are described
and novel approaches to overcome these limitations are introduced. An appropriate combination of expression vectors and optimized
host strains will provide cell factories customized for each production process and expand the great potential of Aspergilli
as biotechnology workhorses to more complex multi-step industrial applications. |
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