The dichotomy of mechanisms of copper accumulation in yeast induced by enhanced levels of copper as well as menadione in growth media |
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Authors: | Felix Funk Walter Schneider |
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Institution: | Institut für Anorganische Chemie, Eidgen?ssische Technische Hochschule Zürich, ETH-Zentrum, CH-8092 Zürich, Switzerland |
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Abstract: | AbstractA high copper accumulation is induced in the yeast Saccharomyces cerevisiae either by menadione at the level of 200 μM in the growth medium, or by elevated concentrations of copper. While the uptake, as well as the toxicity of copper, strongly depend on the zinc concentration in the medium, there is no influence of zinc on copper intake induced by menadione. The copper binding ligand d-penicillamine suppresses the accumulation of copper in the menadione systen, whereas it has virtually no effect in the copper system. Within the range of non-toxic concentrations, copper is predominantly taken up by an energy-dependent mechanism. In contrast, the accumulation in the menadione system is clearly energy-independent. Thus there exist at least two different mechanisms for the uptake of copper in the yeast Saccharomyces cerevisiae. |
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Keywords: | Copper accumulation menadione yeast metal distribution |
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