| 小球藻病毒PBCV-1特异性溶壁酶(Lysin)的溶壁活性 |
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| 引用本文： | 韩继刚,刘平芳,康明,叶寅,田波.小球藻病毒PBCV-1特异性溶壁酶(Lysin)的溶壁活性[J].微生物学报,2002,42(3):354-358. |
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| 作者姓名： | 韩继刚 刘平芳 康明 叶寅 田波 |
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| 作者单位： | [1]河北大学生命科学学院，保定071002 [2]中国科学院微生物研究所，北京100080 |
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| 基金项目： | 国家自然科学基金 ( 39870 0 1 6 )，河北省科技厅攻关计划资助~~ |
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| 摘 要： | 从PBCV－1感染小球藻NC64A的细胞裂解液中提取了Lysin的粗制剂，酶活底物范围分析表明，几丁质酶、壳聚糖酶的β-1,3-葡萄糖苷酶是Lysin活性的主要组成部分，并与小球藻细胞壁的组成甩分相吻合。其中几丁酯酶和壳聚糖酶，特别是几丁酯酶在裂解小球藻细胞壁的过程中发挥了重要的作用。Lysin粗制剂经FPLC分离纯化得到分子量分别为52kD、56kD的两个几丁质酶（Chil和Chi2）和一个分子量为36kD的壳聚糖酶。
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| 关 键 词： | 几丁质酶 壳聚糖酶 β－1 3－葡萄糖苷酶 病毒侵染 小球藻病毒 PBCV－1 特异性溶壁酶 溶壁活性 |
| 文章编号： | 0001-6209(2002)03-0354-05 |
| 修稿时间： | 2001年7月30日 |
The Cell Wall-degrading Activities of Lysin Encoded by Chlorella Virus PBCV-1 |
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| Han Jigang , Liu Pingfang Kang Ming , Yie Yin Tian Po.The Cell Wall-degrading Activities of Lysin Encoded by Chlorella Virus PBCV-1[J].Acta Microbiologica Sinica,2002,42(3):354-358. |
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| Authors: | Han Jigang Liu Pingfang Kang Ming Yie Yin Tian Po |
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| Institution: | College of Life Science, Hebei Universisty, Baoding 071002, China. |
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| Abstract: | Lysin was isolated from Chlorella NC64A lysate which was infected by Chlorella virus PBCV-1. The enzyme property detection of lysin shows that it contains at least three enzyme activities: chitinase, chitosanase and beta-1, 3-Glucanase. This is consistent with the compose of Chlolrella cell wall. Chitinase and chitosanase, especially chitinase plays a important role in the process of virus entry. One 52 kD chitinase, one 56 kD chitosanase and one 36 kD chitosanase were obtained after purified by FPLC. |
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