富硒条件下蜜环菌菌株硒耐受性及胞外酶生物活性的研究

目的]通过对蜜环菌(Armillaria mellea)的富硒驯化,研究各菌株硒耐受性、有机硒含量及生物活性的变化规律,从而获得生物活性更强的蜜环菌,并对富硒蜜环菌的生物学特征进行初步研究.方法]以Na2SeO3为无机硒试剂对蜜环菌进行富硒驯化;采用氢化物原子荧光光谱法测定蜜环菌的硒含量,热水浴法测定蜜环菌的无机硒...

Biological activity and selenium tolerance of Armillaria mellea

Objective] We studied the selenium tolerance, organic selenium content, and biological characteristics of the domesticated Armillaria mellea strains, so as to screen out the strains with strong biological activity. Methods] Na₂SeO₃ was used as the inorganic selenium reagent to domesticate A. mellea. The selenium content and inorganic selenium content of A. mellea was determined by hydride atomic fluorescence spectrometry and hot water bath extraction method, respectively. Excellent A. mellea strains were identified based on morphological, physiological, and biochemical characteristics. Results] A. mellea showed a good ability to accumulate inorganic selenium. The tolerance thresholds of strains J-234, M-H, A-10, and YN-2 to Na₂SeO₃ were 100, 60, 60, 20 mg/L, respectively. The selenium content in strains J-234, M-H, A-10, and YN-2 was 8.378 1, 7.249 4, 1.926 9, 3.606 1 µg/g, respectively. Strain J-234 had the highest biological activity after domestication, with the xylanase activity of 92 522.46 U/L, amylase activity of 11 951.49 U/L, cellulase activity of 8 439.47 U/L, laccase activity of 1.25 U/L, and polypeptide content of 0.087 g/L.Conclusion] The addition of exogenous selenium enhances the biological activity of A. mellea, and this finding provides reference for the selenium domestication of edible and medicinal fungi.